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MAB3194

Anti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH

clone 8.1GSH, Chemicon®, from mouse

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Gabaryty przesyłkiSKUDostępnośćCena netto
100 μg

Przewidywany termin wysyłki29 maja 2026zKuehne + Nagel Sp. z o.o.

1870,00 zł

Informacje o tej pozycji

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
8.1GSH, monoclonal
Technique(s):
ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
Application:
ELISA, FACS, ICC, IHC, WB
Citations:
10

1870,00 zł


Przewidywany termin wysyłki29 maja 2026Szczegóły


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biological source

mouse

Quality Segment

antibody form

purified antibody

antibody product type

primary antibodies

clone

8.1GSH, monoclonal

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

Immunogen

GS-NEM conjugated to KLH

Application

Detect the Glutathione: N-ethylmaleimide adduct protein using this Anti-Glutathione: N-ethylmaleimide adduct, clone 8.1GSH validated for use in ELISA, Flow, ICC, IHC & WB.
Immunohistochemistry: 1:100 (paraformaldehyde fixation)

Immunocytochemistry: 1:100 - 1:500 for assessment of intracellular GSH distribution.

ELISA: 1:100,000 - 1:200,000 to quantify GSH at >2 mM in biological specimens.

Flow cytometry: 1:100 - 1:1,000

Cells were fixed with cold 1% PFA for 5 min and vortexed into methanol (-20°C) containing 5mM NEM (10 min on dry ice) or 10 μM NEM (N-ethyl maleimide) for 30 min at RT.

Optimal working dilutions must be determined by end user.

Positive Control: Peripheral blood mononuclear cells (PBMC) treated with NEM
Research Category
Neuroscience
Research Sub Category
Oxidative Stress

Biochem/physiol Actions

Reactive with thiol-modified glutatione. Does not react with oxidized glutathione (GSSG) or glutathione (GSH) in mixed disulfide linkage with proteins. MAB3194 is specific to the glutathione:N-ethylmaleimide adduct; thus it is useful for quantification of reduced glutathione (GSH) after reaction with N-ethylmaleimide. For use in ELISA or flow cytometry, sample must first be reacted with NEM.

Physical form

Format: Purified
Liquid in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.

Preparation Note

Maintain at 2-8°C in undiluted.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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clone

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biological source

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biological source

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biological source

mouse

biological source

mouse

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified antibody

isotype

IgG1

isotype

IgG1

isotype

IgG1

isotype

IgG1

technique(s)

ELISA: suitable, immunocytochemistry: suitable, western blot: suitable, flow cytometry: suitable, immunohistochemistry: suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100


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Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Numer pozycji handlu globalnego

SKUNUMER GTIN
MAB319404053252265754

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