MAB3118
Anti-DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11
ascites fluid, clone 2EL-1B11, Chemicon®
Synonim(y):
Anti-FLAG Antibody, FLAG Tag Antibody
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 100 μL | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 3010,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352203
NACRES:
NA.43
eCl@ss:
32160702
Clone:
2EL-1B11, monoclonal
Species reactivity:
vertebrates, E. coli
Application:
ELISA, ICC, IF, IP, WB
Citations:
22
Pomoc techniczna
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Pozwól nam pomócbiological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
2EL-1B11, monoclonal
species reactivity
vertebrates, E. coli
species reactivity (predicted by homology)
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
shipped in
dry ice
target post-translational modification
unmodified
General description
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
FLAG® is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
FLAG® is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Immunogen
Epitope: Epitope tag
Synthetic peptide M-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys(C) coupled to ovalbumin
Application
Detect DYKDDDDK (FLAGepitope tag) using this Anti-DYKDDDDK (FLAG epitope tag) Antibody, clone 2EL-1B11 validated for use in ELISA, IC, IF, IP & WB.
Immunofluorescence:
A 1:1,000-1:5,000 dilution of a previous lot was used in IF.
Immunoprecipitation:
A 1:1,000-1:5,000 dilution of a previous lot was used IP on recombinant proteins.
ELISA:
A 1:1,000-1:5,000 dilution of a previous lot was used in Enzyme-linked immunosorbent assay on recombinant proteins.
Note: Does not require Ca++ for binding.
Immunoblotting:
on recombinant proteins
Optimal working dilutions must be determined by the end user.
A 1:1,000-1:5,000 dilution of a previous lot was used in IF.
Immunoprecipitation:
A 1:1,000-1:5,000 dilution of a previous lot was used IP on recombinant proteins.
ELISA:
A 1:1,000-1:5,000 dilution of a previous lot was used in Enzyme-linked immunosorbent assay on recombinant proteins.
Note: Does not require Ca++ for binding.
Immunoblotting:
on recombinant proteins
Optimal working dilutions must be determined by the end user.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Biochem/physiol Actions
DYKDDDDK sequence containing proteins. Reacts with both N-terminal and C-terminal fusion proteins.
Physical form
Ascites mouse monoclonal IgG1 liquid containing no preservative.
Unpurified
Preparation Note
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Cells transfected with FLAG-tagged fusion vector
Cells transfected with FLAG-tagged fusion vector
Routinely evaluated by Western Blot on Flag-mTor protein blot.
Western Blot Analysis:
1:500 dilution of this lot detected Flag mTor on Flag-mTOR blot.
Western Blot Analysis:
1:500 dilution of this lot detected Flag mTor on Flag-mTOR blot.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB3250
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ta pozycja | |||
|---|---|---|---|
| antibody form ascites fluid | antibody form ascites fluid | antibody form purified antibody | antibody form ascites fluid |
| clone 2EL-1B11, monoclonal | clone SIG1-25, monoclonal | clone 1G11, monoclonal | clone 2E8, monoclonal |
| species reactivity vertebrates, E. coli | species reactivity - | species reactivity human | species reactivity rat, human |
| biological source mouse | biological source rabbit | biological source mouse | biological source mouse |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| isotype IgG1 | isotype IgG | isotype IgG2aκ | isotype IgG1κ |
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Klasa składowania
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certyfikaty analizy (CoA)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Qi-Yue Chen et al.
Theranostics, 12(4), 1904-1920 (2022-02-25)
Background: Efforts to prevent recurrence in gastric cancer (GC) patients are limited by current incomplete understanding of the pathological mechanisms. The present study aimed to identify novel tumour metastasis-associated genes and investigate potential value of these genes in clinical diagnosis
Juan Liu et al.
Autophagy, 17(8), 1962-1977 (2020-08-05)
Iron metabolism is involved in numerous physiological processes such as erythropoiesis, oxidative metabolism. However, the in vivo physiological functions of the iron metabolism-related gene Hfe in immune response during viral infection remain poorly understood. Here, we identified 5 iron metabolism-associated
Beate Roese-Koerner et al.
Stem cell reports, 7(2), 207-219 (2016-07-19)
Tight regulation of the balance between self-renewal and differentiation of neural stem cells is crucial to assure proper neural development. In this context, Notch signaling is a well-known promoter of stemness. In contrast, the bifunctional brain-enriched microRNA miR-9/9(∗) has been
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| MAB3118 | 08436037125034 |