Upon addition of Component A and Component B, the final pH should be approximately 7.4.
CC176
Skin dECM Gel Hydrogel Kit
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Informacje o tej pozycji
NACRES:
NA.75
UNSPSC Code:
12352207
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Pomoc techniczna
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Pozwól nam pomócbiological source
Porcine
packaging
pkg of 1 kit
manufacturer/tradename
Sigma-Aldrich
growth mode
N/A
technique(s)
cell culture | mammalian: suitable
shipped in
ambient
storage temp.
2-8°C
General description
The cellular microenvironment directly and indirectly effects cellular behavior via both biophysical and biochemical routes. The microenvironment is composed of extracellular matrix proteins (ECM), other surrounding cells, cytokines, growth factors, hormones, and other bioactive agents and nano/microscale mechanical properties such as physical tension/stiffness. Decellularization is the process used in biomedical engineering to isolate the native extracellular matrix (ECM) of a tissue from its inhabiting cells, leaving an ECM scaffold of the original tissue, which can be used in cell culture and tissue regeneration. Compared to other tumor derived basement membrane extracts (Matrigel@), these dECM hydrogel scaffolds can provide a more physiological environment with increased growth rates for cells without the use of any exogenous growth factors. Our tissue-specific dECM Gel Hydrogel Kits are a series of native decellularized extracellular matrix (ECM) protein solutions derived from a variety of non-diseased porcine organs (bone, heart, liver, kidney, intestine, skin and lung). These hydrogels can be used as traditional 2D ECM coatings or as hydrogels to encapsulate cells for 3D cell culture applications.
Application
pH: 2.0-3.0Gelation: Forms GelBiocompatibility: 90% Cell ViabilitySterility: No Fungi or Bacteria DetectedMycoplasma: NegativePRRS, PRV: Negative
Features and Benefits
Skin decellularized ECM scaffold for traditional 2D and 3D cell culture applications.
Preparation Note
Store all components of the dECM Gel Hydrogel Kit at 2-8°C (do not freeze).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
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pcodes
Hazard Classifications
Met. Corr. 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Klasa składowania
12 - Non Combustible Liquids
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Produkty
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Porównanie hydrożeli ze zdekellularyzowanej macierzy pozakomórkowej (dECM) z matrycami Matrigel® w predykcyjnych modelach hodowli komórkowych 3D
Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)
Comparison of decellularized extracellular matrix (dECM) hydrogels to Matrigel® matrices for predictive 3D cell culture models
Powiązane treści
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| CC176 | 04065265237567 |
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Does pH of the final hydrogel after adding component A, component B and cell suspension is 2.0 - 3.0? If yes, what is the best solution to adjust pH to 6.8 -7.4?
1 answer-
Helpful?
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How much material is one Kit?
1 answer-
The components of this kit are listed on the Product Datasheet, which can be found in the 'DOCUMENTATION' section under 'More Documents':
Skin ECM Solution (CS226373): Qt. 2 (0.3 mL)
Component A (CS226431): Qt. 1 (1 mL)
Component B (CS226430): Qt. 1 (1 mL)Helpful?
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