The production was carried out using a modified method as described in the reference: Timpl, R., et al., J. Biol. Chem. 254:9933-9937 (1979). The nidogen content is not tested, but the product is provided at > 95% purity with primarily 3 chains expressed as two major bands at 200 and 400kD, indicating that the presence of nidogen is likely minuscule, if present at all.
CC095-M
Mouse Laminin
from Engelbreth-Holm-Swarm murine sarcoma basement membrane, liquid, 1 mg/mL, suitable for cell culture
Synonim(y):
Purified Laminin derived for mouse
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 1 mg | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 1590,00 zł | |
| 5 mg | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 4510,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Biological source:
mouse
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Concentration:
1 mg/mL
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócNazwa produktu
Laminin, mouse purified, Laminins are the major noncollagenous constituent of basement membranes. This purified mouse laminin was isolated from Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a mouse tumor that produces large amounts of basement membranes.
biological source
mouse
Quality Level
form
liquid
manufacturer/tradename
Chemicon®
concentration
1 mg/mL
technique(s)
cell culture | mammalian: suitable
input
sample type: human embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type epithelial cells
sample type: mouse embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
-10 to -25°C
General description
Laminin protein is a non-collagenous glycoprotein isolated from Engelbreth-Holm-Swarm (EHS) mouse sarcoma. A laminin protein molecule is composed of three disulfide-linked polypeptides that assemble to form an asymmetrical cross-shaped structure. Purified laminin mouse purified promotes the adhesion and growth of human carcinoma and sarcoma cells, human retinoblastoma cells, liver cells, mouse neuroblastoma cells, mouse embryonal carcinoma cells, and seems to be involved in the development of embryonal tissue. The optimal laminin concentration may vary with the cell type and has to be determined experimentally.
Application
The purified mouse laminin has been used as a coating substrate for culturing and maintaining mouse embryonic stem cell-derived neuronal cultures, providing a suitable surface for cell attachment and growth during in vitro experiments.[1]
Biochem/physiol Actions
Laminin proteins are essential components of the basement membrane which are extracellular matrices holding cells and tissues together. They interact with receptors present in the plasma membrane of cells neighboring basement membranes. This interaction allows laminins to effectively regulate various cellular activities and signaling pathways.
Physical form
Purified from EHS sarcoma according to the method of Timpl, et al.
Purified protein. Liquid; in 0.05 M Tris-HCl, pH 7.4, containing 0.15 M NaCl.
No preservatives.
No preservatives.
Preparation Note
Specificity: Molecular weight = 850 kD; 3 chains expressed as two major bands at 200 and 400 kD on SDS-PAGE.
Preparation: Prepared from Engelbreth-Holm-Swarm {EHS} sarcoma according to the method of Templ et al 1979 {http://www.jbc.org/cgi/reprint/254/19/9933.pdf} Briefly, tumor homogenates were subjected to NaCl extractions and centrifugations. Laminin was found in the 0.5M NaCl fraction. After subsequent salt gradients and further centrifugation steps, the laminin was dialyzed against 2M urea and passed over a column of DEAE, followed by molecular sieve chromatography and concentration by ultrafiltration.
Preparation: Prepared from Engelbreth-Holm-Swarm {EHS} sarcoma according to the method of Templ et al 1979 {http://www.jbc.org/cgi/reprint/254/19/9933.pdf} Briefly, tumor homogenates were subjected to NaCl extractions and centrifugations. Laminin was found in the 0.5M NaCl fraction. After subsequent salt gradients and further centrifugation steps, the laminin was dialyzed against 2M urea and passed over a column of DEAE, followed by molecular sieve chromatography and concentration by ultrafiltration.
Store at -20°C for up to six months.
Thaw product carefully at 2-8°C to avoid premature gelling, and do not refreeze. Once thawed. material should be stored at 2-8°C for up to 3 months.
Thaw product carefully at 2-8°C to avoid premature gelling, and do not refreeze. Once thawed. material should be stored at 2-8°C for up to 3 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| biological source mouse | biological source mouse (Engelbreth-Holm-Swarm (EHS) sarcoma) | biological source mouse (High Concentration) | biological source mouse |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell attachment: suitable, cell differentiation: suitable | technique(s) ELISA: suitable, electron microscopy: suitable, immunoblotting: 0.03-0.06 μg/mL using Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane, immunofluorescence: suitable, immunohistochemistry: 20-40 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tongue sections. |
| concentration 1 mg/mL | concentration - | concentration - | concentration ~1.0 mg/mL |
| Quality Level 100 | Quality Level - | Quality Level - | Quality Level 200 |
| form liquid | form solution | form liquid | form buffered aqueous solution |
| storage temp. -10 to -25°C | storage temp. −20°C | storage temp. - | storage temp. −20°C |
Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 2
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Protokoły
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.
Produkty
Białka macierzy zewnątrzkomórkowej, takie jak laminina, kolagen i fibronektyna, mogą być stosowane jako podłoża do mocowania komórek w hodowli komórkowej.
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
The laminin family
Aumailley M
Journal of Separation Science, 7(1), 48-55 (2013)
G Siano et al.
Journal of molecular biology, 432(24), 166675-166675 (2020-10-16)
Neuronal hyperexcitability linked to an increase in glutamate signalling is a peculiar trait of the early stages of Alzheimer's disease (AD) and tauopathies, however, a progressive reduction in glutamate release follows in advanced stages. We recently reported that in the
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| CC095-5MG-M | 04053252745027 |
| CC095-1MG-M | 04053252626951 |
-
The CoA for this product indicates that it is "Purified as laminin-nidogen complex from EHS sarcoma according to the method of Timpl, et al. (1,2)." The inquiry is whether this is a laminin product or a laminin-nidogen complex.
1 answer-
Helpful?
-