APT171
Caspase 8 Colorimetric Activity Assay Kit, IETD
Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD.
Synonim(y):
FLICE
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About This Item
Kod UNSPSC:
12161503
eCl@ss:
32161000
Polecane produkty
reaktywność gatunkowa (przewidywana na podstawie homologii)
mammals
producent / nazwa handlowa
Chemicon®
metody
activity assay: suitable
numer dostępu NCBI
numer dostępu UniProt
metoda wykrywania
colorimetric
Warunki transportu
dry ice
Opis ogólny
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon′s Caspase-8 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity.
Zastosowanie
Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Komponenty
5X Cell Lysis Buffer (Part No. 90065): 5 mL
5X Assay Buffer (Part No. 90066): 10 mL
Caspase-8 Substrate (Ac-IETD-pNA) (Part No. 90082): 1 mL of 2.5 mg/mL solution
Caspase-8 Inhibitor (Ac-IETD-CHO) (Part No. 90086): 50 μL of 100 μM (0.05 mg/mL) in DMSO
pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
5X Assay Buffer (Part No. 90066): 10 mL
Caspase-8 Substrate (Ac-IETD-pNA) (Part No. 90082): 1 mL of 2.5 mg/mL solution
Caspase-8 Inhibitor (Ac-IETD-CHO) (Part No. 90086): 50 μL of 100 μM (0.05 mg/mL) in DMSO
pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
Przechowywanie i stabilność
Store kit materials at -20°C up to their expiration date.
Special Precautions:
· After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Avoid repeated freeze/thaw cycles.
· The Caspase-8 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
Special Precautions:
· After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Avoid repeated freeze/thaw cycles.
· The Caspase-8 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
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