ABT170

Anti-TUBA1A (TUBA3) Antibody

rabbit polyclonal

• Synonim(y): Tubulin alpha-1A chain, Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: mouse, porcine
• Application: ELISA, ICC, WB
• Citations: 7

Właściwości

• Nazwa produktu: Anti-alpha Tubulin Antibody, nontyrosinated, serum, from rabbit
• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, porcine
• species reactivity (predicted by homology): rat (based on 100% sequence homology), human (based on 100% sequence homology)
• technique(s): ELISA: suitable, immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_001257328
• UniProt accession no.: Q71U36
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TUBA1A(7846)

Opis

General description

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

~51 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human nontyrosinated alpha Tubulin.

Application

Detect Tubulin using this rabbit polyclonal antibody, Anti-alpha Tubulin Antibody, nontyrosinated validated for use in western blotting, ELISA & ICC.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Western Blotting Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin and not tyrosinated alpha Tubulin from purified porcine alpha Tubulin (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).

ELISA Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin, and not nontyrosinated alpha Tubulin in an indirect ELISA and in a competitive ELISA (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin, and not nontyrosinated alpha Tubulin in TC-7 cells in interphase (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).

Biochem/physiol Actions

This antibody detects the nontyrosinated form of alpha Tubulin.

Physical form

Rabbit polyclonal serum in buffer containing serum with 0.05% sodium azide.

Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting in NIH/3T3 cell lysate, which were untreated or treated with Pancreatic Carboxypeptidase (PCA).

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected alpha Tubulin, nontyrosinated in 10 µg of PCA treated NIH/3T3 cell lysate and demonstrated a loss of signal in untreated NIH/3T3 cell lysate.

Other Notes

Replaces: 04-1117

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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