ABN1735

Anti-Oxytocin Receptor

from rabbit, purified by affinity chromatography

• Synonim(y): Oxytocin receptor, OT-R
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: mouse, human
• metody: western blot: suitable
• numer dostępu NCBI: NP_001074616
• numer dostępu UniProt: P97926
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... OXTR(5021) ; mouse ... Oxtr(18430)

Opis

Opis ogólny

Oxytocin receptor (UniProt P97926; also known as OT-R) is encoded by the Oxtr (also known as Otr) gene (Gene ID 18430) in murine species. Oxytocin receptor (OT-R) is widely expressed in the reproductive tract, brain, kidneys, and many other organs, where it mediates the biological functions of oxytocin (OT) in mammals. Best known for their role in lactation and parturition, OT-R and OT meidate a wide variety of physiological functions, including estrous cycle, bone formation, social behaviors, as well as regulation of energy homeostasis, including food intake and thermoregulation. Central administration of OT decreases food consumption in rats, and nesfatin (a satiety hormone) increases OT secretion. Oxt-null and Oxtr-null mice display aberrant thermoregulatory function. In addition, Oxtr-knockout mice have a morphological defect in brown adipose tissue (BAT) cells and develop obesity. Human and murine oxytocin receptors are 7-transmembrane (a.a. 39-63, 75-97, 114-135, 155-175, 203-225, 275-293, 309-331) GPCRs with their N-terminal end at the extracellular side (a.a. 1-38) and C-terminal end at the cytoplasmic side (human a.a. 332-389 and mouse a.a. 332-388).

Specyficzność

This polyclonal antibody specifically stained wild-type, but not Oxtr-knockout, mouse brain tissue sections (Courtesy of Dr. Moses V. Chao, Langone Medical Center, New York University, U.S.A).

Immunogen

BSA-conjugated linear peptide corresponding to a sequence from mouse oxytocin receptor N-terminal extracellular domain.

Zastosowanie

Anti-Oxytocin Receptor Antibody, Cat. No. ABN1735, is a highly specific rabbit polyclonal antibody, that targets Oxytocin Receptor 1 and has been tested in Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A 1:500 dilution from a representative lot detected oxytocin receptor in 10 µg of A431, HEK293, HeLa, Jurkat, MCF-7, and Raji membrane extracts.

Immunofluorescence Analysis: A 1:1000 dilution from a representative lot detected oxytocin receptor immunoreactivity in wild-type, but not Oxtr-knockout, mouse frozen brain tissue sections (Courtesy of Dr. Moses V. Chao, Langone Medical Center, New York University, U.S.A).

Jakość

Evaluated by Western Blotting in human uterus.

Western Blotting Analysis: A 1:500 dilution of this antibody detected oxytocin receptor in 10 µg of human uterus tissue lysate.

Opis wartości docelowych

~55-60 kDa observed. The target band size appears larger than the calculated molecular weight of 42.77/42.81 kDa (human/mouse) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable