ABE501

Anti-phospho IRF3 (ser386) Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): Interferon regulatory factor 3, IRF-3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702

Właściwości

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): rhesus macaque (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_001562
• UniProt accession no.: Q14653
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer386)
• Gene Information: human ... IRF3(3661)

Opis

General description

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity.

~50 kDa observed

Immunogen

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human IRF3 (ser386) near the C-terminus.

Application

Detect IRF-3 using this rabbit polyclonal antibody, Anti-phospho IRF3 (ser386) Antibody validated for use in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected detected IRF3 (ser386) in 10 µg of Calyculin A treated MCF7 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable