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ABE1047

Anti-NRF2a/GABPA Antibody

serum, from rabbit

Synonim(y):

GA-binding protein alpha chain, GABP subunit alpha, Nuclear respiratory factor 2 subunit alpha, Transcription factor E4TF1-60, NRF2a/GABPA

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Informacje o tej pozycji

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ChIP, EMSA, WB
Citations:
5
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Quality Level

100

biological source

rabbit

conjugate

unconjugated

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

technique(s)

ChIP: suitable, electrophoretic mobility shift assay: suitable, western blot: suitable

NCBI accession no.

NP_001184226

UniProt accession no.

Q06546

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GABPA(2551)

General description

GA-binding protein alpha chain (UniProt Q06546; also known as GABP subunit alpha, GABPalpha, NRF-2, Nuclear respiratory factor 2 subunit alpha, Transcription factor E4TF1-60) is encoded by the GABPA (Also known as E4TF1A) gene (Gene ID 2551) in human. Initially identified through their interactions with cytochrome c and cytochrome oxidase promoters, GABPA (NRF-2) and the related nuclear respiratory factor NRF-1 target many nuclear genes essential for mitochondrial respiratory function, including the mitochondrial transcription specificity factors TFB1M and TFB2M. GABPA plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. GABPA is upregulated in response to oxidant exposure or increased cellular reactive oxygen species (ROS), where it mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.
~55 kDa observed

Immunogen

Recombinant protein corresponding to human NRF2a/GABPA.

Application

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected GABPA (NRF-2alpha) occupancy at the TFB1M and TFB2M promoters by ChIP using HeLa chromatin preparations (Gleyzer, N., et al. (2005). Mol Cell Biol. 2005 Feb;25(4):1354-66).
Western Blotting Analysis: A representative lot detected GABPA (NRF-2alpha) in U2OS cell lysates (Gleyzer, N., and Scarpulla, R.C. (2011). J. Biol. Chem. 286(46):39715-39725; Gleyzer, N., and Scarpulla, R.C. (2013). J. Biol. Chem. 288(12):8004-8015).
Electrophoretic Mobility Shift Assay (EMSA) Analysis: A representative lot caused a supershift of GABPA (NRF-2alpha)-DNA complex in EMSA using recombinant GABPA or heparin agarose-purified HeLa nuclear extract and radiolabeled synthetic oligonucleotides corresponding to cytochrome oxidase subunit IV, hTFB1M, or hTFB2M promoter sequence with GABPA recognition sites (Gleyzer, N., et al. (2005). Mol Cell Biol. 2005 Feb;25(4):1354-66).
Electrophoretic Mobility Shift Assay (EMSA) Analysis: A representative lot caused a supershift of GABPA (NRF-2alpha)-DNA complex in EMSA using in vitro translated GABPA and radiolabeled cytochrome oxidase subunit IV promoter fragment containing tandem GABPA recognition sites (Vercauteren, K., et al. (2008). J Biol Chem. 283(18):12102-12111).
Immunoprecipitation Analysis: A representative lot immunoprecipitated GABPA (NRF-2alpha)-PRC complex from human embryonic kidney 293FT cell lysate (Vercauteren, K., et al. (2008). J Biol Chem. 283(18):12102-12111).
This Anti-NRF2a/GABPA Antibody is validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP), Electrophoretic Mobility Shift Assay for the detection of NRF2a/GABPA.

Analysis Note

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected NRF2a/GABPA in 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.
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Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Numer pozycji handlu globalnego

SKUNUMER GTIN
ABE104704055977181562