ABC61-M

Anti-NIPP-1 Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): Nuclear inhibitor of protein phosphatase 1, NIPP-1, Protein phosphatase 1 regulatory inhibitor subunit 8, ARD-1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 300
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human, mouse
• metody: immunocytochemistry: suitable; western blot: suitable
• numer dostępu NCBI: NP_002704
• numer dostępu UniProt: Q12972
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... PPP1R8(5511)

Opis

Opis ogólny

Also known as ARD1 (Activator of RNA decay), NIPP-1 (nuclear inhibitor of protein phosphatase 1) is a ubiquitously expressed multifunctional nuclear scaffold protein of 39 kDa, which plays an important role in integrating transcription, RNA processing, and cell cycle progression. NIPP-1 serves as a signaling hub in the nucleus. Additionally, NIPP-1 contains at least two binding sites for PP1 and is complexed to about one-third of the nuclear pool of PP1. Normally, the NIPP-1 - PP1 complex is inactive, but can be activated following phosphorylation of NIPP-1 with PKA, CFK2, or Src family kinases. NIPP-1 also interacts directly with nucleic acids and components of polycomb repressive complexe 2 (PRC2) and functions as a PRC2-dependent transcriptional repressor.

Specyficzność

This antibody should recognize the alpha and beta isoforms of NIPP-1.

Immunogen

GST-tagged recombinant protein corresponding to human NIPP-1.

Zastosowanie

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NIPP-1 in Raji cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

This NIPP-1 antibody is validated for use in WB & ICC for the detection of the NIPP-1 protein.

Jakość

Evaluated by Western Blot Analysis in Raji nuclear extract.

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected NIPP-1 in 10 µg of Raji nuclear extract.

Opis wartości docelowych

~42 kDa observed. Three isoforms at 39 kDa (alpha isoform), 23 kDa (beta isoform), and 13 kDa (gamma isoform) are known to exist. An uncharacterized band may be observed at ~62 kDa in some nuclear extracts.

Postać fizyczna

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Raji nuclear extract

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids