69670-M
His•Bind® Resin
Synonim(y):
uncharged IDA agarose beads
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
10 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 851,00 zł |
50 mL | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 3360,00 zł |
100 mL | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 5510,00 zł |
Informacje o tej pozycji
NACRES:
NA.56
UNSPSC Code:
41106500
851,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócform
liquid
manufacturer/tradename
Novagen®
storage condition
do not freeze
application(s)
life science and biopharma
storage temp.
2-8°C
General description
His•Bind Resin is an uncharged IDA agarose resin. The binding capacity is approximately 8 mg/ml bed volume. His•Bind Resin is well suited to small to medium scale purifications using either gravity flow columns or batch methods.
Application
HIS-BIND® resin have been used: in purification of HIS TAG® fusion proteins by metal chelation chromatography, for purification of the bacterially produced OCRL1WT/VAR phosphatase domain to study heterogeneity in Lowe Syndrome, in protein purification to study anaerobic phloroglucinol degradation by Clostridium scatologenes, for isolation of His6-tagged proteins for studying the role of human repressor activator protein 1 (RAP1) and glial fibrillary acidic protein (GFAPɛ)proteins in increasing γ-secretase activity in a yeast model system.
Biochem/physiol Actions
HIS-BIND® resin helps in the purification of proteins containing the His-tagged sequence by the process of metal chelation chromatography. It utilizes the charge buffer provided in the HIS-BIND® Buffer Kit, that binds the His-tagged sequence effectively with Ni2+ or Co2+ cations that are immobilized onto the HIS-BIND® resin. The target protein can be easily recovered by eluting with imidazole. The HIS-BIND® resin is designed to be regenerated and reused multiple times, making it a cost-effective solution. Its versatility allows for protein purification under gentle, non-denaturing conditions, or in the presence of either guanidine or urea, depending on the specific requirements of the purification process.
Features and Benefits
- Reusable many times
- Compatible with HIS-BIND® Buffer Kit
- Compatible with 1 mM THP
Legal Information
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Ta pozycja | |||
|---|---|---|---|
| application(s) life science and biopharma | application(s) life science and biopharma | application(s) life science and biopharma | application(s) - |
| form liquid | form slurry | form liquid | form suspension |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename - |
| storage condition do not freeze | storage condition do not freeze | storage condition do not freeze | storage condition - |
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Klasa składowania
3 - Flammable liquids
flash_point_f
86.0 °F
flash_point_c
30 °C
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