The BAEE and Anson units are defined by different enzyme-substrate systems and assay conditions, so a universal conversion is not possible. To relate activities expressed in these different units, both units need to be converted to a common unit, like International Units (IU). Conversion:
International Units (IU) and BAEE units: Approximately 270 BAEE units correspond to 1 International Unit (U) at 25 °C.
International Units (IU) and Anson units: One Anson unit corresponds to 550 IU.
Alternatively, running a side-by-side positive control with known Anson unit activity would allow the determination of the Anson unit activity of an unknown sample.
539125
Protease Assay Kit
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
1 kit | Przewidywany termin wysyłki03 czerwca 2026zKuehne + Nagel Sp. z o.o. | 2900,00 zł |
Informacje o tej pozycji
NACRES:
NA.84
UNSPSC Code:
41116133
2900,00 zł
Przewidywany termin wysyłki03 czerwca 2026Szczegóły
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócusage
sufficient for 200 tests
Quality Segment
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
assay range
sensitivity: 10 ng/mL
input
sample type biological sample(s)
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
General description
A kit suitable for quantitative array of a wide variety of proteases in biological samples, utilizing FTC-casein as a substrate. Enzyme activities that have been detected with this assay kit include: chymotrypsin, elastase, plasminogen, PRONASE® Protease, subtilisin, thermolysin, and trypsin.
This Protease Assay Kit is suitable for the quantitative determination of protease activity in biological samples. This kit has been tested for its suitability in detecting a wide variety of proteases, including serine proteases (trypsin, chymotrypsin, thrombin, plasmin, elastase, subtilisin), cysteine proteases (papain, cathepsin B) and acid proteases (thermolysin, pepsin). Each kit provides sufficient reagents for 200 assays. When used as recommended, this kit is ~100-fold more sensitive than either the casein-gel assay or casein A280 assay. The Protease Assay Kit can detect ~10 ng/ml of protease when a 24 h incubation is used. The sensitivity can be enhanced by up to 3-fold by using a fluorometer (excitation 490 nm and emission 525 nm) rather than a spectrophotometer to detect the proteolytic end products.
Features and Benefits
Assay Time: 3 h
Preparation Note
• FTC-casein should be stored at -20°C and is stable for one year from the date of purchase. FTC-casein stored at 4°C is stable for 2-3 days. Several freeze-thaw cycles will lead to a minor increase in the background fluorescence, so following initial thawing, we suggest that this reagent be divided into 5 aliquots. FTC-casein is light sensitive and should be protected from light.
• Protease positive control should be stored at -20°C. As with many proteases, trypsin is susceptible to autolysis and you may notice a reduction in trypsin activity with time, even if stored at -20°C.
• Incubation buffer and assay buffer may be stored at 4°C or -20°C.
• Protease positive control should be stored at -20°C. As with many proteases, trypsin is susceptible to autolysis and you may notice a reduction in trypsin activity with time, even if stored at -20°C.
• Incubation buffer and assay buffer may be stored at 4°C or -20°C.
Other Notes
A Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
Twining, S.S. 1984. Anal. Biochem.143, 30.
Wiesner, R., et al. 1982. Anal. Biochem.121, 290.
Wiesner, R., et al. 1982. Anal. Biochem.121, 290.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level - |
| input sample type biological sample(s) | input - | input sample type cell lysate | input - |
| assay range sensitivity: 10 ng/mL | assay range - | assay range - | assay range - |
| manufacturer/tradename Calbiochem® | manufacturer/tradename - | manufacturer/tradename Calbiochem® | manufacturer/tradename - |
| detection method colorimetric | detection method - | detection method colorimetric | detection method fluorometric |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
Klasa składowania
10 - Combustible liquids
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
-
Kann ich mit diesem Kit die Anson Units meiner Protease bestimmen?
1 answer-
Helpful?
-
-
Do you have the protocol for 539125-1 using plant extracts?
1 answer-
Unfortunately, there is no specific protocol for use with plant extracts. Please see the link below to review the protocol:
https://www.emdmillipore.com/US/en/product/Protease-Assay-Kit,EMD_BIO-539125#anchor_USPHelpful?
-