524627
Phosphatase Inhibitor Cocktail III
liquid, for the inhibition of serine/threonine and protein tyrosine phosphatases
Synonim(y):
Phosphatase Inhibitor Mix (Set III), Phosphatase inhibitor
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About This Item
Kod UNSPSC:
12352200
NACRES:
NA.77
Polecane produkty
product name
Phosphatase Inhibitor Cocktail Set III, Phosphatase Inhibitor Cocktail Set III is a ready to use cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases.
Postać
liquid
producent / nazwa handlowa
Calbiochem®
warunki przechowywania
OK to freeze
Warunki transportu
wet ice
temp. przechowywania
−20°C
Opis ogólny
Phosphorylation and dephosphorylation regulate signal transduction processes involved in cellular activities such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific amino acid residues (serine, threonine, or tyrosine) on target proteins, changing their conformation and interaction with ligands. Protein phosphatases remove the phosphate group, restoring the protein to its dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain their phosphorylation status intact. Phosphatase Inhibitor Cocktail Set III is a cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials.
Zastosowanie
Phosphatase Inhibitor Cocktail Set III has been used:
- as a supplement in lysis buffer to prepare protein lysates of whole testes for western blotting
- to fortify ice-cold lysis radio-immunoprecipitation assay (RIPA) buffer to homogenize larval hindguts for western blot analysis
- as a component in cell lysis buffer to extract total proteins for western blotting
Działania biochem./fizjol.
Cell permeable: no
Primary Target
Serine/threonine and protein tyrosine phosphatases
Serine/threonine and protein tyrosine phosphatases
Product does not compete with ATP.
Reversible: no
Cechy i korzyści
- Ready-to-use formulations for convenient use
- High-quality products for consistent and reproducible performance,
- Flexible options with a range of cocktails targeting different categories of phosphatases
Komponenty
Each vial contains 1 ml of aqueous solution with the following components:
- 50 mM Sodium Fluoride
- 10 mM ß-Glycerophosphate (Cat. No. 35675)
- 10 mM Sodium Pyrophosphate Decahydrate
- 1 mM Sodium Orthovanadate
Ostrzeżenie
Toxicity: Toxic (F)
Postać fizyczna
In H₂O.
Rekonstytucja
For long-term storage, aliquot and freeze (-20°C). Dilute 1:5 just prior to use.
Informacje prawne
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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