517310

Anti-P-Glycoprotein Mouse mAb (C219)

liquid, clone C219, Calbiochem^®

• UNSPSC Code: 12352203
• Clone: C219, monoclonal
• Species reactivity: rat, hamster, human, mouse
• Application: —
• Citations: 36

Właściwości

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: C219, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: rat, hamster, human, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... ABCB1(5243)

Opis

General description

Purified mouse monoclonal antibody. Recognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.

Recognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.

This Anti-P-Glycoprotein Mouse mAb (C219) is validated for use in FC, Frozen Sections, Immunoblotting, ICC, IP, Paraffin Sections for the detection of P-Glycoprotein.

Immunogen

Hamster and Human

SDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a multidrug resistant human cell line

Application

Flow Cytometry (5-10 µg/1 x 10⁶ cells)

Frozen Sections (5-10 µg/ml)

Immunoblotting (1-10 µg/ml, see application references, see comments)

Immunocytochemistry (see comments)

Immunoprecipitation (see comments)

Paraffin Sections (1-10 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 1% BSA.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Cell lines or frozen tissue, such as liver or colon

Other Notes

Also recognizes ~150 kDa sister P-glycoprotein (SPGP). Frozen sections should be fixed in acetone for 10 min at -20°C. Formalin-fixed, paraffin tissue sections should be deparaffinized and rehydrated by conventional means. Boiling samples may reduce visibility of the protein by immunoblotting (see application references). This antibody is also reported to work for immunocytochemistry and immunoprecipitation. A ~200 kDa protein that migrates in the same position as myosin is also seen by immunoblotting. Variables associated with assay conditions will dictate the proper working dilutions.

Beck, W.T., et al. 1996. Cancer Res. 56, 3010.
Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.

Legal Information

ImmunoblottingLee, G., et al. 2001. J. Pharmacol. Exp. Ther.299, 204.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable