34115-M

N-Ethylmaleimide

≥98% (HPLC), H+-ATPase inhibitor, solid

• Synonim(y): N-Ethylmaleimide, NEM
• Wzór empiryczny (zapis Hilla): C₆H₇NO₂
• Numer CAS: 128-53-0
• Masa cząsteczkowa: 125.13
• UNSPSC Code: 12352111
• MDL number: MFCD00005509
• NACRES: NA.77
• Assay: ≥98% (HPLC)
• Form: solid
• Quality level: 100
• Storage condition: OK to freeze

Właściwości

• Nazwa produktu: N-Ethylmaleimide, Sulfhydryl alkylating reagent that inhibits H⁺-ATPase and suppresses the short circuit current (IC₅₀ = 22 µM) in pancreatic duct cells.
• description: Merck USA index - 14, 3822
• Quality Level: 100
• assay: ≥98% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: ethanol: 5 mg/mL
• storage temp.: 10-30°C
• SMILES string: N1(C(=O)C=CC1=O)CC
• InChI: 1S/C6H7NO2/c1-2-7-5(8)3-4-6(7)9/h3-4H,2H2,1H3
• InChI key: HDFGOPSGAURCEO-UHFFFAOYSA-N

Opis

General description

Sulfhydryl alkylating reagent that inhibits H⁺-ATPase and suppresses the short circuit current (IC₅₀ = 22 µM) in pancreatic duct cells. Inactivates NADP-dependent isocitrate dehydrogenase. Also a potent inhibitor of both Mg²⁺ and Ca²⁺/Mg²⁺-stimulated DNA fragmentation in rat liver nuclei. Stimulates arachidonic acid release through activation of PLA₂ in endothelial cells.

Sulfhydryl alkylating reagent. Has been used for blocking sulfhydryl containing reagents which interfere with glucose oxidase assays. Inactivates NADP-dependent isocitrate dehydrogenase. Endonuclease inhibitor. Also a potent inhibitor of both Mg²⁺ and Ca²⁺/Mg²⁺-stimulated DNA fragmentation in rat liver nuclei.

Biochem/physiol Actions

Primary Target
H+-ATPase

Target IC₅₀: 22 µM in suppressing short circuit current

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Huang, Y.C., et al. 1995. Arch. Biochem. Biophys. 316, 485.
Neve, E.P.A., et al. 1995. Biochem. Pharmacol. 49, 57.
Cain, K., et al. 1994. Biochem. Cell Biol.72, 631.
Banger, K.K., et al. 1993. Biochem. J. 290, 199.
Mattingly, J.R., Jr., et al. 1993. J. Biol. Chem. 268, 3925.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Corrosive (L)

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Informacja o środkach bezpieczeństwa

• pictograms: GHS06,GHS05
• signalword: Danger
• hcodes: H300,H311,H314,H317
• pcodes: P260 - P270 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Oral - Acute Tox. 3 Dermal - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1
• Klasa składowania: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk: WGK 3
• flash_point_f: 163.2 °F - closed cup
• flash_point_c: 72.9 °C - closed cup