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33-016

PI 3-Kinase HTRF^® Assay; 384 wells

Name: PI 3-Kinase HTRF<SUP>®</SUP> Assay; 384 wells
Brand: MM
Price: 3640 PLN
Availability: InStock

The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 1 plate.

Synonim(y):

Assay for PI 3-Kinase, HTRF Assay, PI 3-Kinase Assay

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Gabaryty przesyłki	SKU	Dostępność	Cena netto
1 ea		Przewidywany termin wysyłki21 maja 2026zKuehne + Nagel Sp. z o.o.	3640,00 zł

Informacje o tej pozycji

UNSPSC Code:

12161503

NACRES:

NA.84

eCl@ss:

32161000

3640,00 zł

Przewidywany termin wysyłki21 maja 2026Szczegóły

Pomoc techniczna

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Właściwości

Quality Level

100

species reactivity

human, mouse

manufacturer/tradename

HTRF^®, Upstate^®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_006218.2

UniProt accession no.

P42336

detection method

fluorometric (HTRF)

shipped in

dry ice

Gene Information

human ... PIK3CA(5290)

Opis

Application

Used to detect/quantify: PI 3-Kinase

Biochem/physiol Actions

Target Sub-Family: PI3K/PI4K

Packaging

Kit capacity: 384 wells

Other Notes

4X Reaction Buffer

PIP2 (Substrate; 1mM stock)

Stop Reagent A

Stop Reagent B

DM A Detection Reagent

DM B Detection Reagent

DM C Detection Reagent

Legal Information

HTRF is a registered trademark of Cis Bio International

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Ta pozycja	33-037	33-017	33-036
Sigma-Aldrich 33-016 PI 3-Kinase HTRF^® Assay; 384 wells	Sigma-Aldrich 33-037 PI 3-Kinase HTRF^® Assay; 50,000 wells	Sigma-Aldrich 33-017 PI 3-Kinase HTRF^® Assay; 1920 wells	Sigma-Aldrich 33-036 PI 3-Kinase HTRF^® Assay; 10,000 wells
technique(s) activity assay: suitable (kinase)	technique(s) activity assay: suitable (kinase)	technique(s) activity assay: suitable (kinase)	technique(s) activity assay: suitable (kinase)
Gene Information human ... PIK3CA(5290)	Gene Information human ... PIK3CA(5290)	Gene Information human ... PIK3CA(5290)	Gene Information human ... PIK3CA(5290)
species reactivity human, mouse	species reactivity human, mouse	species reactivity mouse, human	species reactivity mouse, human
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
detection method fluorometric (HTRF)	detection method fluorometric (HTRF)	detection method fluorometric (HTRF)	detection method fluorometric (HTRF)
NCBI accession no. NM_006218.2	NCBI accession no. NM_006218.2	NCBI accession no. NM_006218.2	NCBI accession no. NM_006218.2

pictograms

GHS07

signalword

Warning

hcodes

H319

pcodes

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

Klasa składowania

10 - Combustible liquids

wgk

WGK 2

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PI 3-Kinase HTRF Assay

Complete PI3-Kinase Assay Solution The PI3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for assaying all Class I PI3-Kinases in a homogeneous 384 well format. Nowhere else will you find a kit that offers this level of performance. And, nowhere else you will find as many Class I PI3-Kinases available.

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Enzyme activity effects of N-terminal His-tag attached to catalytic sub-unit of phosphoinositide-3-kinase.

James M J Dickson et al.

Bioscience reports, 33(6) (2013-08-24)

NTT (N-terminal tags) on the catalytic (p110) sub-unit of PI 3-K (phosphoinositol 3-kinase) have previously been shown to increase cell signalling and oncogenic transformation. Here we test the impact of an NT (N-terminal) His-tag on in vitro lipid and protein

A drug targeting only p110α can block phosphoinositide 3-kinase signalling and tumour growth in certain cell types.

Stephen Jamieson et al.

The Biochemical journal, 438(1), 53-62 (2011-06-15)

Genetic alterations in PI3K (phosphoinositide 3-kinase) signalling are common in cancer and include deletions in PTEN (phosphatase and tensin homologue deleted on chromosome 10), amplifications of PIK3CA and mutations in two distinct regions of the PIK3CA gene. This suggests drugs

Oncogenic mutations of p110α isoform of PI 3-kinase upregulate its protein kinase activity.

Christina M Buchanan et al.

PloS one, 8(8), e71337-e71337 (2013-08-13)

In addition to lipid kinase activity, the class-I PI 3-kinases also function as protein kinases targeting regulatory autophosphorylation sites and exogenous substrates. The latter include a recently identified regulatory phosphorylation of the GM-CSF/IL-3 βc receptor contributing to survival of acute

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SKU	NUMER GTIN
33-016	04053252470806

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