233900

Coelenterazine

≥90% (TLC), solid, chemiluminescence probe of the superoxide anion, Calbiochem^®

• Synonim(y): Coelenterazine, 2-( p-Hydroxybenzyl)-6-( p-hydroxyphenyl)-8-benzylimidazo[1,2-a]pyrazin-3-(7H)-one, 2-(p-Hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazo[1,2-a]pyrazin-3-(7H)-one
• Wzór empiryczny (zapis Hilla): C₂₆H₂₁N₃O₃
• Numer CAS: 55779-48-1
• Masa cząsteczkowa: 423.46
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD00467176
• Assay: ≥90% (TLC)
• Form: solid
• Quality level: 100
• Storage condition: OK to freeze, protect from light

Właściwości

• Nazwa produktu: Coelenterazine, A cell-permeable aequorin luminophore that acts as a very sensitive and specific chemiluminescence probe of the superoxide anion.
• Quality Level: 100
• assay: ≥90% (TLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: brown
• solubility: methanol: 1 mg/mL, ethanol: soluble
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: [n]21c(nc([c]2=O)Cc5ccc(cc5)O)c([nH]c(c1)c4ccc(cc4)O)Cc3ccccc3
• InChI: 1S/C26H21N3O3/c30-20-10-6-18(7-11-20)15-23-26(32)29-16-24(19-8-12-21(31)13-9-19)27-22(25(29)28-23)14-17-4-2-1-3-5-17/h1-13,16,27,30-31H,14-15H2
• InChI key: YHIPILPTUVMWQT-UHFFFAOYSA-N

Opis

General description

Coelenterazine (CTZ), a small prosthetic group,[1] is a luciferin or light-producing compound present in luminous marine organisms.[2][3] The imidazopyrazinone ring in CTZ is responsible for its luminous properties.[3] This chemiluminescent dye[4] is soluble in methanol and poorly soluble in water.[1] CTZ can be oxidized with the help of bovine serum albumin (BSA) and several other luciferases.[3]

Application

Coelenterazine - CAS 55779-48-1 – Calbiochem may be used:

• to determine superoxide production[1]
• to determine free radical production[4]
• in cell suspension buffer for in vivo reconstitution of aequorin[5]

Biochem/physiol Actions

Coelenterazine (CTZ) is a cell-permeable aequorin luminophore that acts as a very sensitive and specific chemiluminescence probe of the superoxide anion. It can also be used for detecting changes in intracellular Ca²⁺ in cells that have been transfected with apoaequorin cDNA. CTZ functions as a powerful antioxidant. It serves as a substrate for multidrug resistance protein 1 (MDR1) P-glycoprotein (ATP-binding cassette sub-family B member 1 (ABCB1)/Pgp) and can bind with apoaequorin to form the active aequorin complex.[6] It can be mainly used to quantify the formation of superoxide in neutrophils.[1]

Packaging

Packaged under inert gas

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable