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CELLULYSIN Cellulase, Trichoderma viride

Synonim(y):

β-D-glucosidase, endo-β-1,4 glucanase, cellobiohydrolase

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Informacje o tej pozycji

Numer CAS:
Numer WE:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
eCl@ss:
32160410
Specific activity:
≥10,000 units/g dry wt.
Biological source:
fungus (Trichoderma viride)
Informacje o cenach i dostępności nie są obecnie dostępne.
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biological source

fungus (Trichoderma viride)

form

solid

specific activity

≥10,000 units/g dry wt.

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

technique(s)

transformation: suitable

color

yellow-white

solubility

water: soluble

suitability

suitable for molecular biology

application(s)

food and beverages
pharmaceutical

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Ta pozycja
C2730C061522178
technique(s)

transformation: suitable

technique(s)

-

technique(s)

-

technique(s)

-

biological source

fungus (Trichoderma viride)

biological source

fungus (Trichoderma reesei)

biological source

-

biological source

Aspergillus niger

specific activity

≥10,000 units/g dry wt.

specific activity

≥700 units/g

specific activity

≥5,000 units/g solid

specific activity

~0.8 U/mg

application(s)

food and beverages
pharmaceutical

application(s)

-

application(s)

-

application(s)

-

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

form

solid

form

aqueous solution

form

powder

form

powder

General description

Cellulase includes endo-β-1,4 glucanase, cellobiohydrolase, and β-D-glucosidase. It is commonly produced by bacteria and fungi.[1]

Application

CELLULYSIN Cellulase, Trichoderma viride has been used:
  • to digest transgenic BY-2 cells exposed to paraformaldehyde fixation[2]
  • in the enzyme mix used for in situ hybridization to plant chromosomes
  • in the enzyme solution for the transformation of Phytophthora species[3]
  • to isolate intact cells and protoplasts from plant tissues

Biochem/physiol Actions

Cellulase is useful in food, feed, textile, leather, detergent, and paper industries. It is also applied in fiber modification, biomass fermentation, and pharmaceutical industries.[1]

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 month at -20°C.

Other Notes

One unit is a measure of decomposing activity using a filter paper substrate at 40°C, pH 4.0.
Takebe, I., et al. 1968. Plant Cell Physiol.9, 115.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
CELLULYSIN is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)
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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Klasa składowania

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Weizhen Wang et al.
Frontiers in microbiology, 10, 2402-2402 (2019-11-12)
Phytophthora, a genus of oomycetes, contains many devastating plant pathogens, which cause substantial economic losses worldwide. Recently, CRISPR/Cas9-based genome editing tool was introduced into Phytophthora to delineate the functionality of individual genes. The available selection markers for Phytophthora transformation, however
Farjana Islam et al.
BMC research notes, 11(1), 445-445 (2018-07-06)
This study was conducted to isolate, screening and purification of cellulase from bacteria present in sugar industry waste (molasses) and characterization by morphological and biochemical analysis. Based on experiments, three bacterial strains produced clear transparent zone into carboxymethyl cellulose (CMC)
In Situ Hybridization to Plant Chromosomes
Fluorescence In Situ Hybridization (FISH), 447-494 null
Man-Wah Li et al.
PloS one, 10(8), e0135033-e0135033 (2015-08-14)
Arabidopsis High Mobility Group Box (HMBG) proteins were previously found associated with the interphase chromatin but not the metaphase chromosome. However, these studies are usually based on immunolocalization analysis involving paraformaldehyde fixation. Paraformaldehyde fixation has been widely adapted to preserved
Rahman Ebrahimzadegan et al.
Scientific reports, 9(1), 19989-19989 (2019-12-29)
Here, we characterized the basic properties of repetitive sequences in essential A and supernumerary B chromosomes of Festuca pratensis Huds. This was performed by comparative analysis of low-pass Illumina sequence reads of B chromosome lacking (-B) and B chromosome containing

Numer pozycji handlu globalnego

SKUNUMER GTIN
219466-100KU04055977218466
219466-50KU07790788048723
219466-200KU04055977218473
219466-KU04053252041099

Questions

  1. Does this preparation have activity on beta-glucan polysaccharides other than cellulose, (e.g. xylan, yeast glucan, laminarin, lichenin etc.)?

    1 answer
    1. This enzyme hydrolyzes b1,4-glucan linkages in cellulose. Beta-glucan polysaccharides contain b1,4-glucan linkages.

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