1.15943

Methylene blue (C.I.52015)

for microscopy Certistain^®

• Synonim(y): Methylene Blue trihydrate, Methylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride
• Wzór empiryczny (zapis Hilla): C₁₆H₁₈ClN₃S · 3H₂O
• Numer CAS: 7220-79-3
• Masa cząsteczkowa: 373.90
• EC Index Number: 200-515-2
• UNSPSC Code: 41116130
• Beilstein/REAXYS Number: 3599847
• MDL number: MFCD00012111

Właściwości

• Quality Segment: 400
• form: solid
• potency: 1180 mg/kg LD₅₀, oral (Rat)
• composition: Dye, ≥82% spectrophotometric assay
• pH: 3 (20 °C, 10 g/L in H₂O)
• mp: 190 °C (dec.) (lit.)
• solubility: 50 g/L
• density: 1.757 g/cm³ at 22 °C
• bulk density: 400‑600 kg/m³
• εmax: 2250-2750 at 660-665 nm in 50% ethanol at 0.003 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• SMILES string: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• InChI key: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Opis

General description

Methylene blue (C.I. 52015) for microscopy Certistain^® is a dry dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. Certistain^® is the product group for seleceted high quality dyes that fulfill all quality requirements of IVD and CE registration. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 82 %
Identity
- Chloride:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 660 - 665 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 2250 - 2750
TLC-Test: passes test
Loss on drying (110 °C): 10 - 15 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• pcodes: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable