1.15444

Coomassie Brilliant blue G 250 (C.I. 42655)

for electrophoresis

• Synonim(y): Coomassie Brilliant blue G 250 (C.I. 42655), Acid blue 90, Brilliant Blue G, Cyanine G, Polar Blue G, SERVA BLUE G, protein gel stain
• Wzór empiryczny (zapis Hilla): C₄₇H₄₈N₃NaO₇S₂
• Numer CAS: 6104-58-1
• Masa cząsteczkowa: 854.02
• Numer MDL: MFCD00078482
• Kod UNSPSC: 41105305
• Numer indeksu EC: 228-058-4
• NACRES: NC.01

Właściwości

• Poziom jakości: 100
• siła działania: >5000 mg/kg LD₅₀, oral (Rat)
• metody: protein staining: suitable
• pH: 6.4 (20 °C, 10 g/L in H₂O)
• rozpuszczalność: 40 g/L
• gęstość nasypowa: 520 kg/m³
• temp. przechowywania: no temp limit
• InChI: 1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1
• Klucz InChI: CMGWKYXJIYYESN-UHFFFAOYSA-M

Opis

Opis ogólny

Coomassie Brilliant blue G 250 (C.I. 42655) is a member of Coomassie brilliant blue (CBB), which is a triphenyl methane dye. CBB dyes are widely used for the visualization of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. CBB G-250 (greenish tinted blue) dye is preferably used in Bradford assay for the quantification of proteins.

Zastosowanie

Coomassie Brilliant blue G 250 (C.I. 42655) has been used to stain gel in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It has also been used in Bradford protein assay.

Komentarz do analizy

Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ_max. (buffer pH 7.0): 577 - 584 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.01 g/l; buffer pH 7.0; calc. on dried substance): 450 - 570
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for electrophoresis: passes test

Informacje prawne

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable