1.05290

Agar XLD (wg harm. EP/USP/JP)

GranuCult^® prime, EP, USP, JP, granular, for Salmonella spp., pkg of 500 g

• Synonim(y): Xylose-lysine-deoxycholate agar
• NACRES: NA.74
• UNSPSC Code: 41106200
• Manufacturer/tradename: GranuCult^® prime
• Agency: EP, JP, USP
• Ph: 7.2-7.6 (25 °C, 55.2 g/L in H₂O)
• Form: granular
• Suitability: Salmonella spp.
• Application(s): microbiology; pharmaceutical

Właściwości

• Quality Segment: 200
• agency: EP, JP, USP
• description: Xylose-lysine-deoxycholate agar for microbiology
• sterility: non-sterile
• form: granular
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® prime
• technique(s): microbiological culture: suitable
• color: red
• pH: 7.2-7.6 (25 °C, 55.2 g/L in H₂O)
• solubility: 55.2 g/L
• application(s): microbiology; pharmaceutical
• storage temp.: 15-25°C
• suitability: Salmonella spp.

Opis

General description

XLD Agar (Xylose Lysine Deoxycholate Agar) is a selective and differential medium used for isolating and differentiating pathogenic Enterobacteriaceae, particularly Salmonella and Shigella species. The media consists of yeast extract for vitamins, peptic digest of animal tissue for nitrogenous compounds, and agar as a solidifying agent. Xylose, lactose, and sucrose serve as fermentable carbohydrates. L(+)-lysine detects lysine-decarboxylase activity, causing alkalization and a red-violet color. Sodium thiosulfate and ferric ammonium citrate react with hydrogen sulfide to form black iron sulfide precipitates in colonies. Sodium deoxycholate inhibits coliform bacteria, ensuring selectivity.

Application

XLD Agar has diverse applications across industries. In the food and beverage sector, it is used to detect Salmonella and Shigella in meat, dairy, and processed foods, ensuring compliance with safety standards. In water quality testing, it identifies pathogenic Enterobacteriaceae to ensure the safety of drinking water. The pharmaceutical industry employs XLD Agar for microbial testing to detect contamination in products, adhering to pharmacopeial standards.

Features and Benefits

• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• A high number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Wygląd (klarowność): klarowny
Wygląd (kolor): czerwony
Wartość pH (25 °C): 7,2 - 7,6
Typowy skład (g/litr): Ekstrakt drożdżowy 3,0; Chlorek sodu 5,0; D(+)-Xyloza 3,5; Laktoza 7,5; Sacharoza 7,5; L(+)-Lizyna 5,0; Desoksycholan sodu 2,5; Tiosiarczan sodu 6,8; Cytrynian amonu-żelaza(III) 0.8; Czerwień fenolowa 0,08; Agar-agar 13,5.
Test promocji wzrostu zgodnie ze zharmonizowaną metodą EP, USP i JP.
Inokulum na podłożu referencyjnym (Salmonella typhimurium ATCC 14028 (WDCM 00031)): 10 - 100
Inokulum na podłożu referencyjnym (Salmonella Abony NCTC 6017): 10 - 100
Liczba kolonii (Salmonella typhimurium ATCC 14028 (WDCM 00031)):
Liczba kolonii (Salmonella Abony NCTC 6017):
Odzysk na podłożu testowym (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≥ 50 %
Odzysk na podłożu testowym (Salmonella Abony NCTC 6017): ≥ 50 %
Kolor kolonii (Salmonella typhimurium ATCC 14028 (WDCM 00031)): czerwony
Kolor kolonii (Salmonella Abony NCTC 6017): czerwony
Czarny środek (Salmonella typhimurium ATCC 14028 (WDCM 00031)): +
Czarny środek (Salmonella Abony NCTC 6017): +
Wzrost (Escherichia coli ATCC 8739 (WDCM 00012)): brak do bardzo dobrego
Kolor kolonii (Escherichia coli ATCC 8739 (WDCM 00012)): żółty
Kolor pożywki (Escherichia coli ATCC 8739 (WDCM 00012)): żółty
Osad (Escherichia coli ATCC 8739 (WDCM 00012)): +
Inkubacja: Salmonella 18 godzin; 30-35°C; E.coli do 48 godzin
Powyższe informacje są aktualne w momencie publikacji i mogą ulec zmianie bez powiadomienia (z wyjątkiem klientów posiadających umowę o kontroli zmian z naszą firmą). Informacje/format mogą zostać zmienione w dowolnym czasie i w dowolny sposób, jeśli wymagają tego wewnętrzne sprawy firmy lub standardowe przepisy.

Other Notes

Typical colonies of Salmonella exhibit a black center surrounded by a translucent red zone due to indicator color changes. H₂S-negative Salmonella, such as Salmonella paratyphi A, grow as pink colonies with a dark pink center. The degradation of xylose results in acidification, causing the medium to turn yellow. Strains with lysine-decarboxylase activity convert L(+)-lysine into cadaverine, causing alkalization and a red-violet coloration of the medium. This activity neutralizes the acids produced during xylose degradation, resulting in a red-violet medium. Lactose and sucrose are added to differentiate lysine-decarboxylase-positive coliform bacteria from Salmonella, as their acid formation cannot be neutralized by cadaverine, leaving the medium yellow. Deoxycholate inhibits coliform bacteria growth, while thiosulfate and iron (III) salt react with hydrogen sulfide to form black iron sulfide precipitates in colonies. Sodium thiosulfate is reduced by Salmonella and other sulfate-reducing bacteria to H₂S, which reduces ferric ammonium citrate to black iron sulfide. This reaction is more pronounced under alkaline conditions, but H₂S-producing bacteria that form acids from lactose or sucrose, such as Citrobacter spp. and Proteus spp., may show weaker black centers. Salmonella typhi colonies often appear yellow-orange or pink.

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3