07-371
Anti-Histone H2B Antibody
Upstate®, from rabbit
Synonim(y):
H2B, Histone H2B, H2B histone family, member Q, histone 2, H2be, histone cluster 2, H2be
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
human, chicken
reaktywność gatunkowa (przewidywana na podstawie homologii)
yeast (based on 100% sequence homology), Xenopus (based on 100% sequence homology)
producent / nazwa handlowa
Upstate®
metody
western blot: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
Saccharomyces cerevisiae ... Htb2(852284)
human ... H2BC1(255626)
Opis ogólny
Histone H2B type 3-B (UniProt: Q8N257; also known as H2B type 12) is encoded by the HIST3H2BB gene (Gene ID: 128312) in human. Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone H2B can be monoubiquitinated on Lysine123 by RAD6/UBC2-BRE1 complex to form H2BK123Ub1 complex that gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. H2BK123ub1 is reported to modulates the formation of double-strand breaks during meiosis and is a required for DNA-damage checkpoint activation. Histone H2B is phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase and may be correlated with chromosome condensation. Histone H2B can undergo acetylation by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription.
Specyficzność
This rabbit polyclonal antibody detects Histone H2B in human and chicken. It targets an epitope within 9 amino acids from the C-terminus region.
Immunogen
KLH-conjugated linear peptide corresponding to 9 amino acids from the C-terminus of human Histone H2B type 3-B.
Zastosowanie
Anti-Histone H2B Antibody, Cat. No. 07-371, is a highly specific rabbit polyclonal antibody that targets Histone H2B and has been tested in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Western Blotting Analysis: 1-2 µg/mL from a representative lot detected Histone H2B in acid extract of HeLa cells treated with either sodium butyrate or colcemid.
Jakość
Evaluated by Western Blotting in Chicken Core Histone.
Western Blotting Analysis: 2 µg/mL of this antibody detected Histone H2B in Chicken Core Histone.
Western Blotting Analysis: 2 µg/mL of this antibody detected Histone H2B in Chicken Core Histone.
Opis wartości docelowych
~17 kDa observed; 13.91 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Powiązanie
Replaces: MABE15
Postać fizyczna
Format: Purified
Protein A chromatography
Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, with 0.1% sodium azide and 30% glycerol.
Przechowywanie i stabilność
Stable for 1 year at -20ºC from date of receipt.
Komentarz do analizy
Control
Acid extract from either sodium butyrate or colcemid treated HeLa cells.
Acid extract from either sodium butyrate or colcemid treated HeLa cells.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Certyfikaty analizy (CoA)
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We have shown previously that the pro-inflammatory cytokine TNF (tumour necrosis factor) could drive sLe(x) (sialyl-Lewis(x)) biosynthesis through the up-regulation of the BX transcript isoform of the ST3GAL4 (ST3 β-galactoside α-2,3-sialyltransferase 4) sialyltransferase gene in lung epithelial cells and human
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Nature structural & molecular biology, 19(11), 1108-1115 (2012-10-16)
Promoter-proximal pausing by RNA polymerase II (Pol II) ensures gene-specific regulation and RNA quality control. Structural considerations suggested a requirement for initiation-factor eviction in elongation-factor engagement and pausing of transcription complexes. Here we show that selective inhibition of Cdk7--part of
Produkty
Loading controls in western blotting application.
Kontrole ładowania w aplikacji western blotting.
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