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05-632

Sigma-Aldrich

Anti-14-3-3σ Antibody, clone CS112-2A8

clone CS112-2A8, Upstate®, from mouse

Synonim(y):

Stratifin

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

CS112-2A8, monoclonal

reaktywność gatunkowa

mouse, human, rat

producent / nazwa handlowa

Upstate®

metody

immunoprecipitation (IP): suitable
western blot: suitable

izotyp

IgG1κ

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

dry ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... SFN(2810)
mouse ... Sfn(55948)
rat ... Sfn(313017)

Specyficzność

Recognizes 14-3-3σ, does not cross-react with other 14-3-3 isoforms.

Immunogen

GST fusion protein corresponding to full-length human 14-3-3 sigma

Zastosowanie

Detect 14-3-3σ using this Anti-14-3-3σ Antibody, clone CS112-2A8 validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Jakość

routinely evaluated by immunoblot on RIPA lysates of EGF-stimulated A431 cells

Opis wartości docelowych

30 kDa

Postać fizyczna

Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Przechowywanie i stabilność

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Komentarz do analizy

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Informacje prawne

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

A Santana et al.
Journal of dairy science, 106(9), 6060-6079 (2023-07-21)
The aim of this study was to evaluate the inclusion of alfalfa grazing during 8 h continuous or partitioned in 2 separated sessions of 4 h after each milking, on nutrient intake, digestibility, ruminal fermentation, feeding behavior, milk production, milk
Claudio A Pozo et al.
Journal of dairy science, 105(6), 4987-5003 (2022-04-23)
Our aim was to evaluate whether increasing soluble carbohydrates in the herbage by changing the time of the grazing session or including Acacia mearnsii tannin in the diet would affect intake, digestion, N partitioning, and productive performance of dairy cows
D E Oliveira et al.
Journal of dairy science, 95(3), 1437-1446 (2012-03-01)
Feeding conjugated linoleic acid (CLA) in a rumen-inert form to dairy ewes has been shown to increase milk production, alter milk composition, and increase the milk fat CLA content. However, few studies have tested ruminally unprotected CLA sources. The objective
Jonas de Souza et al.
Journal of dairy science, 100(2), 1072-1085 (2016-12-13)
The objective of our study was to investigate the effects of sources of calcium salts of fatty acids (FA) on production, nutrient digestibility, energy balance, and carryover effects of early lactation grazing dairy cows. Treatment diets were offered from 3
J Zha et al.
The Journal of biological chemistry, 272(39), 24101-24104 (1997-09-26)
BAD interacts with anti-apoptotic molecules BCL-2 and BCL-XL and promotes apoptosis. BAD is phosphorylated on serine residues in response to a survival factor, interleukin-3. Phosphorylated BAD cannot bind to BCL-XL or BCL-2 at membrane sites and is found in the

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