LIG2

QuickLink^™DNAライゲーションキット

joins blunt-end and sticky-end DNA fragments

• 別名: DNA Ligation Kit, Ligation Kit
• UNSPSC Code: 12352200
• EC Number: 232-770-0
• NACRES: NA.55

特性

• grade: Molecular Biology
• Quality Segment: 200
• form: liquid
• usage: kit sufficient for 50 ligation reactions
• technique(s): molecular cloning: suitable
• shipped in: dry ice
• storage temp.: −70°C

詳細

General description

Sigma′s DNA Ligation Kit contains all of the reagents necessary to perform DNA ligation reactions at room temperature using blunt or sticky ends. This kit replaces previous workflows requiring cooking steps and long incubations. Pre-made buffers allow for fast & easy setup.

Application

QuickLink^™ DNA Ligation Kit has been used for the ligation of pGL2-basic luciferase-reporter plasmid with vascular endothelial growth factor (VEGF) promoter sequence and for the ligation of NLR family caspase activating and recruitment domain (CARD)-containing protein 4 (Nlrc4) promoter with the luciferase plasmid vector pGL4.10-luc2

The QuickLink^™ DNA Ligation Kit is suitable for:

• Joining blunt or cohesive-end fragments of DNA into a cloning vector
• Recircularization of linear DNA
• Formation of concatamers
• dsDNA nick repair

Biochem/physiol Actions

One of the most important steps in the cloning process is the ligation of linear DNA into a cloning vector. DNA ligations are performed by incubating DNA fragments with appropriately linearized cloning vectors in the presence of buffer, ATP, and DNA ligase. Many parameters affect ligations such as the relative ratio of insert to vector, the quality and type of the DNA ends, the temperature of ligation and the concentration of DNA.

Features and Benefits

• わずか5分でライゲ-ションします。
• 室温で操作可能、冷却装置は不要です。
• 高いライゲ-ション効率(ライゲ-ション産物を有する形質転換コロニ-数で測定)。
• 制限酵素処理したインサ-トやPCR産物の、平滑末端・突出末端へのライゲ-ションに最適化されています。
• ライゲ-ション反応液から直接、細菌の形質転換が行えます。
• プラスミド・ファ-ジにおけるクロ-ニング、リンカ-(アダプタ-)付加、直鎖状DNAの再環状化、コンカテマ-化に適しています。

Other Notes

Sufficient for 50 reactions:

• 500uL 2X Ligation Buffer A (L9537)
• 100uL 5X Ligation Buffer B (L9662)
• 250 units T4 DNA Ligase (D2886) in 50% glycerol with 10 mM Tris-HCl (pH 7.5) 50 mM KCl, and 1 mM DTT

Legal Information

QuickLink is a trademark of Sigma-Aldrich Co. LLC

安全性情報

• 保管分類: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• wgk: WGK 1