CRISPR・遺伝子編集

関連製品資料

• Article: Cas9-Mediated Recombineering Enhances Metabolic Production of Kdo2-Lipid A by E. coli

  In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either ssDNA or dsDNA as an editing template.

• CRISPR使用許諾契約

  This Product and its use are the subject of one or more of the following patents controlled by The Broad Institute, Inc. (BROAD), the Massachusetts Institute of Technology (MIT), or the President and Fellows of Harvard College (HARVARD): U.S. Patent Nos. 8,697,357; 8,771,945, and any substitutions, divisions, continuations, reissues, renewals, re-examinations or extensions, and corresponding foreign patent applications and patents.

• Article: Ribonucleoprotein (RNP) Protocols Using Synthetic sgRNAs and Cas9 proteins

  Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.

• Article: Genome Editing in Plants with CRISPR/Cas9

  Zinc finger nucleases (ZFNs), were introduced by Sigma-Aldrich less than 8 years ago, but in that time the technology of targeted genome editing has advanced rapidly. Most recently, the discovery of the CRISPR/Cas9 pathway has accelerated interest in this field, opening up new possibilities for research and development.

• eBook: Gene Editing 101

  Learn how to understand, design and perform CRISPR experiments, as well as design gRNA, choose a Cas9 format, screen with CRISPR, use advanced CRISPR formats, and much more…

• Article: CRISPR Cas9 Protein Product Selection Guide

  A convenient CRISPR Cas9 protein selection guide to assist with your CRISPR RNP, lentivirus, mRNA, plasmids, vectors and control needs.

• Article: Blue-White Screening & Protocols for Colony Selection

  Our popular blue-white screening for colony selection technical resource hub, transfection reagent selection guide and protocols.

• PCR Master Mix Calculator

  Ensure the success of your reactions at any scale with our handy PCR Master Mix Calculator resource tool.

• Article: Introduction to Cell Transfection

  An overview on cell transfection vs transduction methods, our cell transfection reagent selection guide and protocols.

• Protocol: Western Blotting

  Western blotting protocol that includes technical information supporting the following: Sample preparation, membrane transfer/tank transfer, transfer stack assembly, buffers, immunodetection, and a troubleshooting guide.