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Merck

A4675

Sigma-Aldrich

Agar

Type E, suitable for plant cell culture

Synonim(y):

Agar-agar, Gum agar

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About This Item

Wzór liniowy:
(C12H18O9)n
Numer CAS:
Numer WE:
Numer MDL:
Kod UNSPSC:
50405901
eCl@ss:
42040102
NACRES:
NA.21

pochodzenie biologiczne

algae (Rhodophyceae)

typ

Type E

Postać

powder

metody

cell culture | plant: suitable

temp. przejścia

transition temp 32-38 °C (1.5%)

Zastosowanie

agriculture

temp. przechowywania

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

Klucz InChI

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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Opis ogólny

Agar or Type E agar is a thermo-reversible, polysaccharide gelling agent. Structurally it consists of two main fractions about 70% of agarose and 30% of agaropectin. The gelling properties of agar comes from the agarose, the high gel strength backbone consists of a linear polymer of 3-linked β-D-galactose and 4-linked 3,6-anhydro-α-L-galactose (LA) repeating units.
Agar or Type E agar is a thermo-reversible, polysaccharide gelling agent. Structurally, it consists of two main fractions about 70% of agarose and 30% of agaropectin. The gelling properties of agar comes from the agarose, the high gel strength backbone consists of a linear polymer of 3-linked ß-D-galactose and 4-linked 3,6-anhydro-α-L-galactose (LA) repeating units.

Zastosowanie

Agar has been used as reagent for the following studies:
  • Plant cell culture studies.
  • As medium supplement for the growth of wild-type Arabidopsis thaliana (ecotype Columbia) and transgenic seeds.
  • For the solidification of culture medium of cork oak somatic embryos.
  • to examine its effect on metal(loid) sensitivity of the Arabidopsis thaliana metal-hypersensitive mutants
Agar suspension culture may be used for the analysis of cell transformation of palyoma virus. It might have been used for incubation of single antibody-forming cell to investigate the plaque formation.
Agar has been used to examine its effect on metal(loid) sensitivity of the Arabidopsis thaliana metal-hypersensitive mutants.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


Certyfikaty analizy (CoA)

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Pere Puigderrajols et al.
Journal of experimental botany, 53(373), 1445-1452 (2002-05-22)
The timing and tissue localization of small heat shock proteins (sHSPs) during cork oak somatic embryo development was investigated under normal growing culture conditions and in response to stress. Western blot analyses using polyclonal antibodies raised against cork oak recombinant
AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS.
I MACPHERSON et al.
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Journal of cellular physiology, 76(1), 77-84 (1970-08-01)
Selection of Agar Reagents for Medium Solidification Is a Critical Factor for Metal (loid) Sensitivity and Ionomic Profiles of Arabidopsis thaliana
Uraguchi S, et al.
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