A1047

Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat

Name: Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat
Brand: SIGMA
Price: 2350 PLN

affinity isolated antibody, buffered aqueous solution

Synonim(y):

Goat Anti-Mouse IgG (γ-chain specific)−AP

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1 ML

2350,00 zł

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Informacje o tej pozycji

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162632

Conjugate:

alkaline phosphatase conjugate

Clone:

polyclonal

Application:

ELISA (d), WB

Citations:

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Pozwól nam pomóc

biological source

goat

recombinant

expressed in goat

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

technique(s)

direct ELISA: 1:7,000-1:21,000, western blot: suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

200

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Secondary Antibodies

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Ta pozycja	A2429	A9688	A4937
Sigma-Aldrich A1047 Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A2429 Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A9688 Anti-Mouse IgM (μ-chain specific)–Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A4937 Anti-Mouse IgA (α-chain specific)−Alkaline Phosphatase antibody produced in goat
technique(s) direct ELISA: 1:7,000-1:21,000, western blot: suitable	technique(s) direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40, western blot (chemiluminescent): 1:160,000-1:320,000	technique(s) direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000	technique(s) direct ELISA: 1:30,000, western blot: 1:30,000
species reactivity mouse	species reactivity mouse	species reactivity mouse	species reactivity -
biological source goat	biological source goat	biological source goat	biological source goat
conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat anti-mouse IgG (gamma-chain specific)-alkaline phosphatase antibody associates with mouse IgG (gamma-chain specific).

Immunogen

purified mouse IgG

Application

Antibodies to Trypanosoma cruzi were detected in the sera of infected mice by ELISA with an alkaline phosphatase conjugated goat anti-mouse IgG chain specific. Plates were coated with T. cruzi antigen and blocked with 1% BSA/PBS prior to incubation with conjugated antibody.

For detection of IgG antibodies in mouse sera and saliva following S. gordonii infection, ELISA assays were performed using anti-mouse IgG alkaline phosphatase antibody at 1:20000. This antibody did not detect IgA antibodies.

Goat anti-mouse IgG (gamma-chain specific)-alkaline phosphatase antibodies can be used for western blot assays,.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Klasa składowania

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number

0000474483

0000479666

SLCM1182

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Florian Krammer et al.

Journal of virological methods, 167(1), 17-22 (2010-03-23)

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Minimizing fucosylation in insect cell-derived glycoproteins reduces binding to IgE antibodies from the sera of patients with allergy.

Dieter Palmberger et al.

Biotechnology journal, 9(9), 1206-1214 (2014-04-23)

The baculovirus/insect cell system has proven to be a very powerful tool for the expression of several therapeutics. Nevertheless, these products sometimes suffer from reduced biological activity and unwanted side effects. Several studies have demonstrated that glycosylation can greatly influence

Rodent host specificity of European hantaviruses: evidence of Puumala virus interspecific spillover.

J Klingström et al.

Journal of medical virology, 68(4), 581-588 (2002-10-12)

In order to investigate rodent host specificity of European hantaviruses, experimental infection of colonized and wild-trapped rodents was performed. In addition to the natural rodent reservoir, Clethrionomys glareolus, Puumala hantavirus (PUUV) could infect colonized Microtus agrestis and Lemmus sibiricus, but

Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta.

Lu Fan et al.

ACS omega, 7(33), 28932-28945 (2022-08-30)

While many proteins are known clients of heat shock protein 90 (Hsp90), it is unclear whether the transcription factor, thyroid hormone receptor beta (TRb), interacts with Hsp90 to control hormonal perception and signaling. Higher Hsp90 expression in mouse fibroblasts was

Absence of ATM truncations in patients with severe acute radiation reactions.

R A Clarke et al.

International journal of radiation oncology, biology, physics, 41(5), 1021-1027 (1998-08-27)

Severe acute toxicity limits the effective use of radiotherapy in patients who are radiosensitive, and it is not usually possible to identify these radiohypersensitive (R-H) individuals before treatment commences. Five such R-H patients were detected over a 3-year period. We

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