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Sigma-Aldrich

Imidazole buffer Solution

BioUltra, 1 M in H2O

Synonim(y):

Imidazole solution, Glyoxaline solution

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About This Item

Wzór empiryczny (zapis Hilla):
C3H4N2
Numer CAS:
288-32-4
Masa cząsteczkowa:
68.08
Beilstein:
103853
Numer MDL:
MFCD00005183
Kod UNSPSC:
12161700
Identyfikator substancji w PubChem:
57652144
NACRES:
NA.25

linia produktu

BioUltra

Postać

liquid

stężenie

1 M in H2O

zanieczyszczenia

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

pH

9.5±0.5

gęstość

1.01 g/mL at 20 °C

ciąg SMILES

c1c[nH]cn1

InChI

1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)

Klucz InChI

RAXXELZNTBOGNW-UHFFFAOYSA-N

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Opis ogólny

Imidazole is used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for the assay of horseradish peroxidase and as a chelator for the binding of various divalent cations. Imidazole aids the elution of histidine-containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for the detection of proteins.

Zastosowanie

Imidazole buffer solution has been used as a component:
  • of calmodulin-binding buffer 1 (CBB1) for the tandem affinity purification (TAP) of TAP-tagged proteins together with co-purifying proteins
  • of lysis buffer, and SDS loading buffer for His-ubiquitin pull-down assays
  • of imidazole wash and elution buffer for protein purification

Piktogramy

Health hazardCorrosion
GHS08,GHS05

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H314,H360D

Klasyfikacja zagrożeń

Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

Kod klasy składowania

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certyfikaty analizy (CoA)

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