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Merck

16-232

Przeciwciało anty-α-Tubulina, klon DM1A, koniugat Alexa Fluor™ 488

clone DM1A, Upstate®, from mouse

Synonim(y):

Tubulina alfa 3, Tubulina B-alfa-1, Tubulina łańcuch alfa-3, tubulina, alfa 1a, tubulina, alfa 3, tubulina, alfa, specyficzna dla mózgu

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Wybierz wielkość

100 μG

2710,00 zł

2710,00 zł

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Informacje o tej pozycji

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR™ 488
Clone:
DM1A, monoclonal
Application:
FACS, ICC, IF, WB
Citations:
17

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Pozwól nam pomóc

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

DM1A, monoclonal

species reactivity

rat, mouse, guinea pig, human, pig, bovine, avian

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

NP_006000

UniProt accession no.

Q71U36

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TUBA1A(7846)

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Ta pozycja
05-82905-829-AF64705-829-AF555
Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 488 conjugate clone DM1A, Upstate®, from mouse

Sigma-Aldrich

16-232

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 488 conjugate

Anti-α-Tubulin Antibody, clone DM1A clone DM1A, Upstate®, from mouse

Sigma-Aldrich

05-829

Anti-α-Tubulin Antibody, clone DM1A

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 647 conjugate clone DM1A, from mouse, ALEXA FLUOR™ 647

Sigma-Aldrich

05-829-AF647

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 647 conjugate

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 555 Conjugate clone DM1A, from mouse, ALEXA FLUOR™ 555

Sigma-Aldrich

05-829-AF555

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 555 Conjugate

conjugate

ALEXA FLUOR™ 488

conjugate

-

conjugate

ALEXA FLUOR™ 647

conjugate

ALEXA FLUOR™ 555

Gene Information

human ... TUBA1A(7846)

Gene Information

human ... TUBA1A(7846)

Gene Information

human ... TUBA1A(7846)

Gene Information

-

species reactivity

rat, mouse, guinea pig, human, pig, bovine, avian

species reactivity

avian, pig, rat, bovine, mouse, human, guinea pig

species reactivity

guinea pig, rat, porcine, human, gerbil, mouse, bovine, avian

species reactivity

guinea pig, gerbil, mouse, bovine, avian, human, rat, porcine

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

clone

DM1A, monoclonal

clone

DM1A, monoclonal

clone

DM1A, monoclonal

clone

DM1A, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

General description

50-60 kDa
Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.

Immunogen

Epitope: The epitopehas been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).
Purified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).

Application

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate is an antibody against α-Tubulin for use in FC, IC, IF & WB.
Western Blot Analysis:
2 µg/mL of a previous lot detected α-Tubulin in RIPA lysates from A431 cells.

Immunocytochemistry:
2 µg/mL of a previous lot showed positive immunostaining for α-Tubulin in HeLa cells.

Biochem/physiol Actions

Recognizes α-Tubulin, clone DM1A, Mr 50-60 kDa.

Physical form

Alexa Fluor™ 488 conjugated, purified mouse monoclonal IgG1 in buffer containing PBS containing 1% BSA, 0.05% Tween-20, 0.05% sodium azide.
Liquid at 2-8°C.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
Do Not Freeze. Do not store the material diluted.
For maximum recovery of product, centrifuge original vial prior to removing cap.

Analysis Note

Control
Negative Control: Catalog # 16-240, Normal Mouse IgG, Alexa Fluor™ 488-conjugate.
Routinely evaluated by Flow Cytometry.

Flow Cytometry:
0.2 μg of this lot detected α-Tubulin in fixed and permeabilized rat L6, Jurkat, and A431 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Simon Schneider et al.
eLife, 12 (2023-11-28)
Cylicins are testis-specific proteins, which are exclusively expressed during spermiogenesis. In mice and humans, two Cylicins, the gonosomal X-linked Cylicin 1 (Cylc1/CYLC1) and the autosomal Cylicin 2 (Cylc2/CYLC2) genes, have been identified. Cylicins are cytoskeletal proteins with an overall positive
Naila Umer et al.
Frontiers in cell and developmental biology, 9, 749559-749559 (2021-12-07)
Profilins (PFNs) are key regulatory proteins for the actin polymerization in cells and are encoded in mouse and humans by four Pfn genes. PFNs are involved in cell mobility, cell growth, neurogenesis, and metastasis of tumor cells. The testes-specific PFN3
Yin-Wei Kuo et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(12), 5533-5541 (2019-03-07)
The remodeling of the microtubule cytoskeleton underlies dynamic cellular processes, such as mitosis, ciliogenesis, and neuronal morphogenesis. An important class of microtubule remodelers comprises the severases-spastin, katanin, and fidgetin-which cut microtubules into shorter fragments. While severing activity might be expected
Wei-Ting Yueh et al.
Development (Cambridge, England) (2021-12-15)
Aneuploidy is frequently observed in oocytes and early embryos, begging the question of how genome integrity is monitored and preserved during this critical period. SMC3 is a subunit of the cohesin complex that supports genome integrity, but its role in
Atsuko Kubo et al.
The Journal of comparative neurology, 527(5), 985-998 (2018-11-09)
Tau is a microtubule-associated protein (MAP) that is localized to the axon. In Alzheimer's disease (AD), the distribution of tau undergoes a remarkable alteration, leading to the formation of tau inclusions in the somatodendritic compartment. While the abnormal aggregated tau
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Questions

  1. What is the recommended dilution for flow cytometry, the product page states "0.2 μg of this lot detected α-Tubulin in fixed and permeabilized rat L6 . . . cells". Is this a 1:500 dilution (eg 100 ug / 0.2ug per test)?

    1 answer

    1. The datasheet from a recent lot, which includes the information you requested, indicates that the antibody is supplied at a concentration of 0.5 mg/mL in a total volume of 200 µL, providing 100 µg of antibody per vial.

      Based on our recommended protocol of using 0.2 µg of antibody per ~2.5 × 10⁵ cells, each vial is expected to provide approximately 500 tests. However, please note that optimization may be required depending on the specific cell type and any variations in cell number or assay volume used in your workflow.

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