P4591
ピルビン酸オキシダーゼ 細菌
lyophilized powder, ≥1.5 U/mg
別名:
ピルビン酸:酸素オキシドレダクターゼ(リン酸化)
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| 容量 | カタログ番号 | 在庫状況 | 単価 |
|---|---|---|---|
| 100 units | カートの在庫状況を確認する | ￥46,800 |
この商品について
CAS番号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-644-5
MDL number:
Specific activity:
≥1.5 U/mg
￥46,800
カートの在庫状況を確認する
1 of 1
当該品目 | |||
|---|---|---|---|
| specific activity ≥1.5 U/mg | specific activity ≥35 units/mg protein (biuret) | specific activity ≥50 units/mg protein | specific activity 15-30 units/mg protein (biuret) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| mol wt ~260 kDa | mol wt - | mol wt 64 kDa | mol wt tetramer ~32 kDa |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
Isoelectric point : 4.3
Michaelis constant : 3.4 X 10-4M (Pyruvate)
Inhibitors : Fe++,Zn++,Cu++,Ag+,Hg++
Optimum pH : 5.7
Optimum temperature : 65oC
pH Stability : pH 5.7 - 6.5 (25oC, 20hr)
Thermal stability : below 45oC (pH 6.0, 15min)
Michaelis constant : 3.4 X 10-4M (Pyruvate)
Inhibitors : Fe++,Zn++,Cu++,Ag+,Hg++
Optimum pH : 5.7
Optimum temperature : 65oC
pH Stability : pH 5.7 - 6.5 (25oC, 20hr)
Thermal stability : below 45oC (pH 6.0, 15min)
Application
Pyruvate Oxidase from microorganisms has been used in the Amplex Red-based fluorescence assay for pyruvate. It has also been used in developing near real-time continuous detection system for pyruvate.
Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and CO2. It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarumand Streptococcus pneumoniae. Pyruvate Oxidase is used for enzymatic determination of pyruvate, GOT, and GPT in clinical analysis.
Biochem/physiol Actions
Pyruvate Oxidase oxidises pyruvate to form acetate, hydrogen peroxide and carbondioxide. Pyruvate oxidase requires flavin adenine dinucleotide (FAD), thiamine pyrophosphate (TPP) and magnesium as cofactors for its catalytic activity. Pyruvate oxidase is activated by the cofactor thiamine.
Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.
Physical form
凍結乾燥品(安定剤としてFADと糖を含有)
Other Notes
1ユニットは、pH 5.7、37°Cでピルビン酸とリン酸がアセチルリン酸とCO2へと変換される反応において、1分間で、1.0 μmolのH2O2を生成する酵素量です。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Masoom Yasinzai
The International journal of biological markers, 24(2), 107-111 (2009-07-28)
Cancer patients are found to have elevated serum levels of lipid-associated sialic acid (LASA). LASA measurement provides a valuable test for the diagnosis of human cancer. The currently used measurement methods are tedious and nonselective. Here we report a method
Piotr Neumann et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(45), 17390-17395 (2008-11-08)
The thiamin- and flavin-dependent peripheral membrane enzyme pyruvate oxidase from E. coli catalyzes the oxidative decarboxylation of the central metabolite pyruvate to CO(2) and acetate. Concomitant reduction of the enzyme-bound flavin triggers membrane binding of the C terminus and shuttling
A novel biosensor based on activation effect of thiamine on the activity of pyruvate oxidase
Akyilmaz E and Yorganci E
Biosensors And Bioelectronics, 23(12), 1874-1877 (2008)
Lanyan Zheng et al.
Journal of bacteriology, 193(2), 516-526 (2010-11-03)
Streptococcus sanguinis is a commensal oral bacterium producing hydrogen peroxide (H₂O₂) that is dependent on pyruvate oxidase (Spx) activity. In addition to its well-known role in bacterial antagonism during interspecies competition, H₂O₂ causes cell death in about 10% of the
Alice Dawson et al.
Journal of molecular biology, 401(2), 253-264 (2010-07-06)
The first committed step in the classical biosynthetic route to menaquinone (vitamin K(2)) is a Stetter-like conjugate addition of alpha-ketoglutarate with isochorismate. This reaction is catalyzed by the thiamine diphosphate and metal-ion-dependent 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD). The medium-resolution (2.35 A) crystal
プロトコル
Protocol for Assay Procedure for Pyruvate Oxidase Bacterial
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| P4591-100UN | 04061834374909 |
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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