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詳細
Research area: Cell Signaling
Alcohol Oxidase (AOX) is a homo-octamer composed of eight flavin adenine dinucleotide (FAD) cofactors and belongs to the glucose-methanol-choline (GMC) family of oxidoreductases. The AOX1 and AOX2 genes are responsible for encoding AOX.[1] Alcohol oxidase is primarily localized in the peroxisome but is also found in the cytoplasm.[2] Alcohol oxidase is a 600 kDa homooctomeric flavoprotein with eight equal 74 kDa subunits; each containing a flavin adenine dinucleotide (FAD) molecule.[3]
Alcohol Oxidase (AOX) is a homo-octamer composed of eight flavin adenine dinucleotide (FAD) cofactors and belongs to the glucose-methanol-choline (GMC) family of oxidoreductases. The AOX1 and AOX2 genes are responsible for encoding AOX.[1] Alcohol oxidase is primarily localized in the peroxisome but is also found in the cytoplasm.[2] Alcohol oxidase is a 600 kDa homooctomeric flavoprotein with eight equal 74 kDa subunits; each containing a flavin adenine dinucleotide (FAD) molecule.[3]
アプリケーション
Alcohol oxidase has been used:
- to catalyze the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes [2].
- to study methanol metabolism in yeasts, such as Candida, Pichia, and Hansenula.
- to study protein translocation into peroxisomes.
- for the determination of ethanol concentration in alcoholic drinks using enzymatic assay.[4]
- in development of enzyme electrode for the determination of alcohols.[5]
生物化学的／生理学的作用
Alcohol oxidase has the highest affinity for methanol. The affinity decreases with increasing chain length of the alkyl (R) group. The enzyme shows little activity toward secondary, tertiary, or aromatic alcohols; or aliphatic alcohols with a chain length of more than 5 carbons. The pH range for activity of this product is 6.5-8.5, with the optimum pH being 7.5. Alcohol oxidases facilitate the conversion of alcohol into carbonyl compounds while producing hydrogen peroxide.[1] It is also the first enzyme in the yeast methanol utilization pathway.[1]
単位の定義
1ユニットは、pH 7.5、25°C、1分間で、1.0 μmolのメタノールをホルムアルデヒドに酸化する酵素量です。
物理的形状
リン酸カリウムバッファ-塩、DTEおよび安定化剤を含有します。
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A6941-BULK:
A6941-10UN:
A6941-50UN:
A6941-50UN-PW:
A6941-250UN-PW:
A6941-VAR:
A6941-250UN:
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試験成績書（COA）
Lot/Batch Number
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H Gülce et al.
Biosensors & bioelectronics, 17(6-7), 517-521 (2002-04-18)
A new enzyme electrode for the determination of alcohols was developed by immobilizing alcohol oxidase in polvinylferrocenium matrix coated on a Pt electrode surface. The amperometric response due to the electrooxidation of enzymatically generated H(2)O(2) was measured at a constant
Erol Akyilmaz et al.
Talanta, 61(2), 113-118 (2008-10-31)
An amperometric biosensor based on catalase enzyme for alcohol determination was developed. To construct the biosensor catalase was immobilized by using gelatin and glutaraldehyde on a Clark type dissolved oxygen (DO) probe covered with a teflon membrane which is sensitive
Alcohol oxidase from Candida boidinii.
H Sahm et al.
Methods in enzymology, 89 Pt D, 424-428 (1982-01-01)
H R Waterham et al.
The Journal of cell biology, 139(6), 1419-1431 (1998-02-12)
Alcohol oxidase (AOX), the first enzyme in the yeast methanol utilization pathway is a homooctameric peroxisomal matrix protein. In peroxisome biogenesis-defective (pex) mutants of the yeast Pichia pastoris, AOX fails to assemble into active octamers and instead forms inactive cytoplasmic
B Vinet
Clinical chemistry, 33(12), 2204-2208 (1987-12-01)
This method for the specific determination of methanol in serum is based on the following two reactions: (formula; see text) Alcohol oxidase is not specific: it converts all lower alcohols to their corresponding aldehydes; however, formaldehyde dehydrogenase is specific and
プロトコル
To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.
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