D9693

抗ダブルコルチン ウサギ宿主抗体

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: 抗DCX抗体, 抗LISX抗体, 抗XLIS抗体, 抗X染色体連鎖脳回欠損抗体, 抗二重皮質抗体
• UNSPSC Code: 12352203
• NACRES: NA.43
• MDL number: MFCD03454961
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 10

特性

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~40 kDa
• species reactivity: human, mouse, rat
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1 mg/mL
• technique(s): western blot: 1-2 μg/mL using HEK-293T cells expressing human doublecortin. Also, mouse and rat brain extract (S1 fraction).
• UniProt accession no.: O43602
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DCX(1641) ; mouse ... Dcx(13193) ; rat ... Dcx(84394)

詳細

Immunogen

ヒトダブルコルチンのアミノ酸151~170に相当する合成ペプチドのKLH結合体。この配列はラットおよびマウスのダブルコルチン内の配列と同一です。

Application

Anti-Doublecortin antibody produced in rabbit has been used in immunoblotting and immunohistofluorescence.[1]

Anti-Doublecortin antibody produced in rabbit is suitable for immunoblotting at a working concentration of 1-2μg/mL using HEK-293T cells expressing human doublecortin, and mouse brain and rat brain extract (S1 fraction).

Biochem/physiol Actions

Doublecortin is a 40kDa microtubule-associated protein (MAP) that is essential for migration of neurons during the development of the cerebral cortex. Mutations in the human DCX gene cause lissencephaly (smooth brain) or subcortical laminar heterotopia (SCLH), characterized by mental retardation and seizures. DCX interaction with MAPs stabilizes microtubules (MTs). The interaction with MTs is via a conserved N-terminal doublecortin (DC) domain. DCX can be regulated by phosphorylation at multiple sites by several kinases, including JNK, Cdk5, PKA, and MARK/PAR-1 family of protein kinases.

Physical form

0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)