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由来生物
rabbit
品質水準
結合体
unconjugated
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
フォーム
buffered aqueous solution
分子量
antigen ~40 kDa
化学種の反応性
human, rat, mouse
強化検証
recombinant expression
Learn more about Antibody Enhanced Validation
濃度
~1.5 mg/mL
テクニック
western blot: 0.5-1 μg/mL using HEK-293T cells expressing human doublecortin and rat brain extract (S1 fraction)
western blot: 1-2 μg/mL using mouse brain extract (S1 fraction)
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... DCX(1641)
mouse ... Dcx(13193)
rat ... Dcx(84394)
詳細
Doublecortin gene is mapped to human chromosome Xq23. It comprises two homologous doublecortin (DC) domains, one each in the N-terminal and C-terminal region. A linker connects a flexible unstructured region. The C-terminal sequences also have serine/proline-rich region.
特異性
Anti-Doublecortin(N-terminal) specifically recognizes human, rat, and mouse Doublecortin.
免疫原
ラットダブルコルチンのN末端に位置するアミノ酸1~17に相当する合成ペプチドのKLH結合体。この配列はマウスにおいて同一であり、ヒトダブルコルチンでも高度に保存されています。
アプリケーション
Anti-Doublecortin antibody produced in rabbit is suitable for immunoblotting at a working concentration of 0.5-1μg/mL using HEK-293T cells expressing human doublecortin and rat brain extract (S1 fraction), and 1-2μg/mL using mouse brain extract (S1 fraction).
生物化学的／生理学的作用
Doublecortin is a 40kDa microtubule-associated protein (MAP) that is essential for migration of neurons during the development of the cerebral cortex. Mutations in the human DCX gene cause lissencephaly (smooth brain) or subcortical laminar heterotopia (SCLH), characterized by mental retardation and seizures. DCX interaction with MAPs stabilizes microtubules (MTs). The interaction with MTs is via a conserved N-terminal doublecortin (DC) domain. DCX can be regulated by phosphorylation at multiple sites by several kinases, including JNK, Cdk5, PKA, and MARK/PAR-1 family of protein kinases.
物理的形状
0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)
保管および安定性
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
D9943-200UL:
D9943-VAR:
IXO14256:
D9943-25UL:
D9943-BULK:
最新バージョンのいずれかを選択してください：
Cui-Hong Zhou et al.
Frontiers in cellular neuroscience, 13, 275-275 (2019-07-12)
Electroacupuncture (EA) pretreatment is a clinically useful therapy for several brain disorders. However, whether and via which exact molecular mechanisms it ameliorates post-traumatic stress disorder (PTSD) remains unclear. In the present study, rats received EA stimulation for seven consecutive days
Orly Reiner et al.
Cell cycle (Georgetown, Tex.), 3(6), 747-751 (2004-05-01)
The mammalian cortex is generally subdivided into six organized layers, which are formed during development in an organized fashion. This organized cortical layering is disrupted in case of mutations in the doublecortin (DCX) gene. DCX is a Microtubule Associated Protein
D Horesh et al.
Human molecular genetics, 8(9), 1599-1610 (1999-08-11)
X-linked lissencephaly is a severe brain malformation affecting males. Recently it has been demonstrated that the doublecortin gene is implicated in this disorder. In order to study the function of Doublecortin, we analyzed the protein upon transfection of COS cells.
J G Gleeson et al.
Neuron, 23(2), 257-271 (1999-07-10)
Doublecortin (DCX) is required for normal migration of neurons into the cerebral cortex, since mutations in the human gene cause a disruption of cortical neuronal migration. To date, little is known about the distribution of DCX protein or its function.
Thomas V Wuttke et al.
Nature neuroscience, 21(4), 517-529 (2018-03-07)
Repair of complex CNS circuitry requires newly incorporated neurons to become appropriately, functionally integrated. One approach is to direct differentiation of endogenous progenitors in situ, or ex vivo followed by transplantation. Prior studies find that newly incorporated neurons can establish
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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