C4231

モノクローナル抗β-カテニン, リン酸化型(pSer³³/pSer³⁷)抗体 マウス宿主抗体

clone BC-22, purified from hybridoma cell culture

• 別名: Anti-CTNNB, Anti-EVR7, Anti-MRD19, Anti-NEDSDV, Anti-armadillo
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD03457566
• Conjugate: unconjugated
• Clone: BC-22, monoclonal
• Application: ARR, ELISA (i), WB
• Citations: 20

特性

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: BC-22, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~94 kDa
• species reactivity: mouse, human, rat, chicken
• concentration: ~2 mg/mL
• technique(s): indirect ELISA: suitable, microarray: suitable, western blot: 5-10 μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132
• isotype: IgG2b
• UniProt accession no.: P35222
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer33/pSer37)
• Gene Information: human ... CTNNB1(1499) ; mouse ... Ctnnb1(12387) ; rat ... Ctnnb1(84353)

詳細

General description

CTNNB1 (catenin β 1) gene is mapped to human chromosome 3p21. The encoded protein β-catenin is localized to the nucleus.

Immunogen

ヒトβ-カテニンのセリン33および37を含む領域に相当する合成リン酸化ペプチド。

Application

Monoclonal Anti-phospho-β-Catenin (pSer33/pSer37) antibody is suitable for:

• indirect ELISA
• microarray[1]
• western blot at a dilution of 5-10μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132.

Biochem/physiol Actions

pSer³³ ペプチドよりもpSer³⁷ペプチドの方が効果的に抗体を阻害し、非リン酸化ペプチドの場合は阻害されません。非リン酸化型またはSer³³リン酸化型のタンパク質は検出されません。また、リン酸化型プラコグロビンも認識しません。

β-catenin is mapped on the short arm of chromosome 3. It is useful for differential diagnosis of Osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. It may serve as a prognostic marker for CCA progression and hence can be significant in the CCA therapy target.
In Wnt/β-catenin signal pathway β-Catenin participates as a signal transduction molecule. The pathway is associated with development, tissue homeostasis and oncogenic transcription. Upregulation of the gene is associated with glioma cell proliferation. The protein encoded has dual function that regulates the coordination of cell-cell adhesion by linking cytoskeleton and cellular junctions and gene transcription. Wnt/β-catenin signal pathway is known to induce progression colon cancer progression.

Physical form

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable