A274

モノクロナール抗H⁺/K⁺ ATPアーゼ (β サブユニット) マウス宿主抗体

clone 2G11, ascites fluid, buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD02096517
• Conjugate: unconjugated
• Clone: 2G11, monoclonal
• Application: IF, IHC (f), WB CL
• Citations: 4

特性

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 2G11, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 60-80 kDa (glycosylated form)
• species reactivity: pig, mouse, rabbit, rat, bovine, ferret, canine
• technique(s): immunohistochemistry (frozen sections): suitable, indirect immunofluorescence: 1:2,000, western blot (chemiluminescent): 1:4,000
• isotype: IgG1
• UniProt accession no.: P18597
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Atp4b(11945) ; rat ... Atp4b(24217)

詳細

General description

抗体は、さまざまな生物種のH⁺/K⁺ ATPア-ゼβサブユニットと反応します。βサブユニットの細胞質側に位置するアミノ酸1-13または15-28内に存在するエピト-プと結合します。抗体は、H⁺/K⁺-ATPア-ゼの酵素活性を阻害するとともに、細胞質のK⁺結合部位の親和性を変化させることが確認されています。H⁺/K⁺ ATPア-ゼβサブユニットの存在の特定および検出に利用することができます。

The H⁺/K⁺-ATPase is a gastric proton pump that is a member of the P-type cation-transporting adenosine 5′-triphosphatase (ATPase) family. H⁺/K⁺-ATPase consists of a large transmembrane, catalytic α subunit, that contains sites for ATP binding and phosphorylation. This ATPase also has a smaller glycoprotein (β) subunit which may play a role in maintaining the structural and functional integrity of the complex. Mouse monoclonal anti-H⁺/K⁺ ATPase (β subunit) binds to H⁺/K⁺-ATPase β subunit in cow, dog, pig, rabbit, mouse, ferret and rat tissues. By immunoblotting, the antibody detects various forms of the β subunit, including a 60-80kDa glycosylated protein, a 52kDa β subunit precursor and the 34kDa core peptide.

Immunogen

脱グリコシル化したブタ胃ミクロソ-ム由来H⁺/K⁺ ATPア-ゼのコアを形成する34 kDa βサブユニット。

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal anti-H⁺/K⁺ ATPase (β subunit) antibody can be used for indirect immunofluorescence (1:2,000), chemiluminescent western blot (1:4,000), and immunohistochemical applications.

Physical form

PBS溶液 (0.05%アジ化ナトリウム含有)。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)