A275

モノクロナール抗Na⁺/K⁺ ATPアーゼ (α1 サブユニット) マウス宿主抗体

clone 9A-5, ascites fluid

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD01633896
• Conjugate: unconjugated
• Clone: 9A-5, monoclonal
• Application: ELISA (i), IHC (f)
• Citations: 12

特性

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 9A-5, monoclonal
• mol wt: antigen ~110 kDa
• species reactivity: rat, human, canine, avian
• technique(s): immunohistochemistry (frozen sections): 1:1,000, indirect ELISA: suitable
• isotype: IgG1
• UniProt accession no.: P05023
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ATP1A1(476) ; rat ... Atp1a1(24211)

詳細

General description

エピト-プマッピングの研究により、抗体は細胞内領域でαサブユニットのAsp-369またはその付近に結合することが示唆されています。染色は、Na⁺/K⁺ ATPア-ゼの形質膜における局在性と一致しています。

Mouse Monoclonal Anti-Na⁺/K⁺ ATPase (α1 Subunit) antibody localizes the Na⁺/ K⁺ ATPase α1 subunit in human, canine, rat and avian tissues.

Na⁺/K⁺ transporting ATPase subunit α1 is an ion transport pump critical for maintaining the gradient of Na and K ions across the plasma membrane.

The gene coding Na⁺,K⁺-ATPase is mapped to human chromosome 1p13.1, which is a plasma membrane ion transporter. It is ubiquitously expressed in all animals. The α subunit of the protein consists of 10 transmembrane helices, and three cytoplasmic domains A, N and P that serve as actuator, in nucleotide-binding and for phosphorylation, respectively.

Immunogen

ラット腎臓Na⁺/K⁺ ATPア-ゼ。

Application

Monoclonal Anti-Na⁺/K⁺ ATPase (α1 Subunit) antibody produced in mouse has been used in immunohistochemistry.

Mouse Monoclonal Anti-Na⁺/K⁺ ATPase (α1 Subunit) antibody has been used for immunohistochemical assays. It has also been used for immunolabeling assays to quantify the expression of proteins involved in cellular ion transport. Furthermore, the product is suitable for use in indirect ELISA.

Biochem/physiol Actions

Na⁺,K⁺-ATPase facilitates the transport of Na⁺ and K⁺ ions across plasma membrane in an ATP-dependent manner. Therefore, it is responsible for maintaining ion homeostasis across the cell and controls cellular excitability in electrically active tissues. The Na⁺ potential established by Na⁺,K⁺-ATPase is required for the function of secondary active transporters. Variation in the gene mostly results in protein function alteration, otherwise in some cases its stability, expression or plasma membrane targeting property is affected. Mutation in the gene is known to cause a number of disease conditions including Familial Hemiplegic Migraine type 2, Rapid-onset Dystonia Parkinsonism and Alternating Hemiplegia of Childhood. Na⁺,K⁺-ATPase serves as a receptor for cardiotonic steroids, that play a vital role in triggering proinflammatory cytokine production, in chronic inflammatory diseases, such as atherosclerosis.

Physical form

PBS溶液 (0.05%アジ化ナトリウム含有)。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)