L2012

リポポリサッカリド サルモネラ菌 セロタイプ enteritidis由来

purified by gel-filtration chromatography

• 別名: LPS
• UNSPSC Code: 12352201
• NACRES: NA.25
• MDL number: MFCD00131520

特性

• biological source: Salmonella enterica (Serotype enteritidis)
• Quality Segment: 200
• form: lyophilized powder
• purified by: gel-filtration chromatography
• impurities: <1% Protein
• color: white to faint yellow
• solubility: water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow
• shipped in: ambient
• storage temp.: 2-8°C

詳細

General description

This product is extracted from Salmonella enteritidis and purified by gel filtration. The source strain is ATCC 13076. Lipopolysaccharide from Salmonella enteritidis is mutagenic in murine spleen cells and causes a plaque-forming response in those cells.

Application

リポ多糖類（LPS）はグラム陰性細菌の細胞壁に特徴的な成分です。LPS とその脂質A部分は、Toll 様受容体 4（TLR4）による自然免疫系の細胞を刺激します。

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

安全性情報

• 保管分類: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable