E0885
エンテロキナーゼ ブタ腸由来
lyophilized powder, ≥100 units/mg protein
別名:
エンテロペプチダーゼ
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
40 units | カートの在庫状況を確認する | ￥42,800 |
200 units | カートの在庫状況を確認する | ￥174,000 |
この商品について
CAS番号:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-761-1
NACRES:
NA.54
MDL number:
Specific activity:
≥100 units/mg protein
form
lyophilized powder
Quality Segment
specific activity
≥100 units/mg protein
mol wt
150 kDa
purified by
chromatography
composition
Protein, ≥20% Lowry
foreign activity
aminopeptidase ≤1.5%, trypsin ≤1%, free
storage temp.
−20°C
Application
Enterokinase from porcine intestine has been used in a study to investigate complementary DNA cloning and sequencing of rat enteropeptidase. Enterokinase from porcine intestine has also been used to learn more about the insulinotropic region of the gastric inhibitory polypeptide.
The enzyme from Sigma has been used to activate zymogens in order to detect trypsin activity. The study to investigated the structural and evolutionary consequences of unpaired cysteines in trypsinogen.[1] The product has been used to measure trypsin while studying the effect of pesticide induced alterations in gene expression in the lobster, Homarus americanus[2] The enzyme from Sigma has been used to develop a novel assay for measuring levels of lipid-free apoA-I in the presence of lipid-bound apoA-I. Enteropeptidase can specifically cleave human lipid-free apoA-I but not its lipid-bound form resulting in an N-terminal fragment of 22 kDa.[3] It has also been used in a study to examine the effect of calcium and phytic acid on the activation of trypsinogen and the stability of trypsin.
Biochem/physiol Actions
Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.
Physical form
凍結乾燥品(リン酸ナトリウムバッファ-塩含有)
Other Notes
1ユニットは、pH 5.6、25°C、1分間で、1.0 nmolのトリプシンをトリプシノーゲンから生成する酵素量です。
1 of 1
当該品目 | |||
|---|---|---|---|
| specific activity ≥100 units/mg protein | specific activity ≥0.5 units/mg solid | specific activity - | specific activity ≥4.0 units/mg protein |
| form lyophilized powder | form salt-free, lyophilized powder | form powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 150 kDa | mol wt 150 kDa | mol wt 150 kDa (consisting of 115kDa and 35kDa subunits.) | mol wt 25.9 kDa |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| purified by chromatography | purified by - | purified by - | purified by - |
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保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)