D9905
デンハルト溶液, 凍結乾燥粉末
for Northern and Southern blotting, lyophilized powder
別名:
Denhardt′s Solution For Blotting Technique
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
5 mL | カートの在庫状況を確認する | ￥23,400 |
5 x 5 mL | カートの在庫状況を確認する | ￥78,800 |
この商品について
NACRES:
NA.25
UNSPSC Code:
12161700
￥23,400
カートの在庫状況を確認する
高品質はそのまま、さらにお手頃な価格になりました
grade
Molecular Biology
Quality Segment
form
lyophilized powder
technique(s)
Southern blotting: suitable
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
2-8°C
General description
Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.
Application
Suitable as a blocking Buffer for Southern and Northern blot membranes.
Denhardt′s Solution has been used:
- as a component in hybridization buffer for the hybridization of brain sections
- in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) process
- in the preparation of hybridization mixture[1]
Biochem/physiol Actions
Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.[2]
Preparation Note
水5mLで復元すると50倍濃縮液となります。
Each vial of Denhardt′s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).
1 of 1
当該品目 | |||
|---|---|---|---|
| technique(s) Southern blotting: suitable | technique(s) Southern blotting: suitable | technique(s) - | technique(s) - |
| grade Molecular Biology | grade - | grade - | grade Molecular Biology |
| Quality Level 100 | Quality Level 300 | Quality Level 100, 200 | Quality Level 200 |
| form lyophilized powder | form solution | form liquid (2x concentrate) | form solution |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. -10 to -25°C | storage temp. room temp |
| foreign activity DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected | foreign activity DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected | foreign activity DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected | foreign activity - |
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保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)