D9893
pBR322 Plasmid DNA from E. coli RRI
lyophilized powder
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
1 unit | カートの在庫状況を確認する | ￥77,300 |
5 units | カートの在庫状況を確認する | ￥309,000 |
この商品について
CAS番号:
UNSPSC Code:
12352200
eCl@ss:
32160414
EC Number:
297-227-2
NACRES:
NA.52
MDL number:
￥77,300
カートの在庫状況を確認する
高品質はそのまま、さらにお手頃な価格になりました
biological source
Escherichia coli
Quality Segment
grade
Molecular Biology
form
lyophilized powder
mol wt
2.9 MDa, 4363 bp
storage temp.
2-8°C
General description
Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in E. coli. This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives. Plasmid pBR322 confers resistance to ampicillin and tetracycline. The plasmid sequence has been published.
The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I).
The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I).
Application
Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in Escherichia coli. This plasmid and derivatives have been used for a number of purposes including cloning, selection and expression of recombinant molecules, construction of shuttle vectors and vectors for nucleotide sequencing, studies of elements involved in gene expression, as plasmid DNA standards, and as a model system for studies on prokaryotic plasmid replication.
pBR322 Plasmid DNA from E. coli RRI has been used as a low molecular weight DNA to test the enzymatic activity of partially purified component from Perna viridis (Linn.)[1] It has also been used for investigating its effect on the calcium carbonate particle morphology by scanning electron microscopy studies[2]
Biochem/physiol Actions
Unique Sites: Within the gene for ampicillin resistance: Pst I, Pvu I, Sca I; Within the gene for tetracycline resistance: BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, Xma III; other unique sites: Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, Tth111 I.
One of the most commonly used cloning vectors, pBR322, confers resistance to ampicillin and tetracycline. The DNA sequence of the entire plasmid has been published.
This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives.
Other Notes
Accession number: J01749
Accession number: J01749
DNA is provided as a powder, lyophilized from a solution of 1 mM Tris-HCl (pH 7.5) with 1mM NaCl and 1mM EDTA.
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当該品目 | |||
|---|---|---|---|
| form lyophilized powder | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| mol wt 2.9 MDa, 4363 bp | mol wt 2.9 MDa, 4363 bp | mol wt - | mol wt - |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade Molecular Biology |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| biological source Escherichia coli | biological source - | biological source - | biological source Escherichia coli (RRI) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
D9893-5UN: + D9893-.25UN: + D9893-PH: + D9893-VAR: + D9893-1UN-PW: + D9893-5UN-PW: + D9893PROC: + D9893-BULK-N: + D9893-1UN: + D9893-BULK:
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