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MABE1007

Anti-TEL-AML1 fusion Antibody, clone 6F2

Name: Anti-TEL-AML1 fusion Antibody, clone 6F2
Brand: MM

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容量	カタログ番号	在庫状況	単価

この商品について

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Clone:

monoclonal

Species reactivity:

human

Application:

ChIP, FACS, WB

Citations:

現在、価格および在庫状況を閲覧できません。

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特性

biological source

rat

Quality Level

300

clone

monoclonal

species reactivity

human

technique(s)

ChIP: suitable, flow cytometry: suitable, western blot: suitable

詳細

General description

The chimeric transcription factor TEL-AML1 (ETV6-RUNX1) is the result of the reciprocal translocation t(12;21)(p13;q22), the most common structural genomic alteration found in childhood B-cell precursor acute lymphoblastic leukaemia (ALL). The runt-homology DNA-binding domain of AML1 retained in the TEL-AML1 mediates the DNA-binding activity of the fusion protein. The fusion protein not only exhibits altered transcription activity, it also affects normal transcription activity by directly competing against wild-type AML1 for promoter binding, sequestering transcriptional cofactors available for the wild-type protein, as well as dimerizing with wild-type protein.

Immunogen

KLH-conjugated linear peptide corresponding to human TEL-AML1 fusion.

Application

Anti-TEL-AML1 fusion Antibody, clone 6F2 is an antibody against TEL-AML1 fusion for use in Western Blotting, Flow Cytometry, Chromatin Immunoprecipitation .

Flow Cytometry Analysis: A representative lot detected TEL-AML1 fusion induction upon mifepristone treatment in BA/F3 cells expressing inducible TEL-AML1 fusion (Linka, Y., et al. (2013). Blood Cancer J. 3:e151).
Chromatin Immunoprecipitation Analysis

Analysis Note

Evaluated by Western Blotting in lysate from mifepristone-treated murine BA/F3 cells expressing inducible TEL-AML1 fusion.
Western Blotting Analysis: A 1:20,000 dilution of this antibody detected TEL-AML1 fusion induction in 2.5 ug lysate from mifepristone-treated murine BA/F3 cells expressing inducible TEL-AML1 fusion.

Other Notes

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

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当該品目	MABE145	MABN683	HPA000264
Sigma-Aldrich MABE1007 Anti-TEL-AML1 fusion Antibody, clone 6F2	Sigma-Aldrich MABE145 Anti-Runx3 Antibody, clone R3-5G4	Sigma-Aldrich MABN683 Anti-ETV5 Antibody, clone 3H3	Sigma-Aldrich HPA000264 Anti-ETV6 antibody produced in rabbit
clone monoclonal	clone R3-5G4, monoclonal	clone 3H3, monoclonal	clone polyclonal
biological source rat	biological source mouse	biological source mouse	biological source rabbit
species reactivity human	species reactivity human	species reactivity human	species reactivity human
technique(s) ChIP: suitable, western blot: suitable, flow cytometry: suitable	technique(s) immunoprecipitation (IP): suitable, western blot: suitable	technique(s) western blot: suitable	technique(s) ChIP: 1-10 μg (per reaction), immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:200-1:500
Quality Level 300	Quality Level 100	Quality Level 100	Quality Level 100

試験成績書（COA）

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Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System

A major focus of breast cancer research is to understand the mechanisms responsible for disease progression and drug resistance. Toward that end, it has been found that approximately two thirds of all human breast carcinomas overexpress the Estrogen Receptor α (ERα) protein and it remains the primary pharmacological target for endocrine therapy1,2. The normal cellular function of ERα is as a transcription factor that mediates a wide variety of physiological processes, many of which are dependent upon phosphorylation of the receptor at specific amino acid residues3,4. Indeed, ERα is known to be phosphorylated at a multitude of different sites, yet how these all correlate to disease remains unclear5. Here, we interrogated multiple sites of ERα for phosphorylation status by screening an extensive panel of different breast cancer patient samples and other non-breast cancer tissue microarray (TMA) slide samples to determine their relevance to disease.

NPI Flyer- Epigenetics and Nuclear Function Feature

Historically chromatin was considered to contain only DNA, histones, non-histone proteins and transiently associated mRNAs. While this remains true, current data suggest that a variety of non-coding RNAs (e.g. long non-coding RNAs, enhancer RNAs and even miRNAs) also associate with chromatin and serve regulatory functions.

グローバルトレードアイテム番号

カタログ番号	GTIN
MABE1007	04055977168259

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