The gelation process for this product does not require pH neutralization by the addition of acid or base. The procedure involves pipetting undiluted collagen into culture dishes, exposing them to ammonia vapor, and rinsing with sterile distilled water multiple times to remove ammonia. The final rinse is replaced with a sterile medium or balanced salt solution with serum, and the dishes are stored at 4°C until use.
08-115
Rat Collagen Type I
from rat tail, liquid, 2-5 mg/mL, suitable for cell culture, used for 3D gel formation
別名:
ラット尾コラーゲン, ラット尾コラーゲンタイプ I
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| 容量 | カタログ番号 | 在庫状況 | 単価 |
|---|
この商品について
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Biological source:
rat
Form:
liquid, solution
Technique(s):
cell culture | mammalian: suitable (and for 3D proliferation assays.)
Concentration:
100 mg
現在、価格および在庫状況を閲覧できません。
製品名
I型コラーゲン、ラット尾部, 100 mg, solution, BioReagent, suitable for cell culture
biological source
rat
Quality Level
form
liquid, solution
manufacturer/tradename
Upstate®
concentration
100 mg
technique(s)
cell culture | mammalian: suitable (and for 3D proliferation assays.)
input
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type: mouse embryonic stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
Gene Information
rat ... COL1A1(29393)
General description
ヒトでは、I型コラーゲンがコラーゲンの中で最も豊富に存在し広く発現しています。このヘテロ三量体には、2つのα1 (I)鎖と1つのα2 (I)鎖が含まれています。[1]I型コラーゲンα 1（COL1A1）はI型コラーゲンの主要な成分であり、I型コラーゲンのpro-α 1鎖をコードします。[2]これは、Gly-Xaa-Yaaトリペプチド配列の反復を有します。コラーゲンα1(I)鎖タンパク質は、コラーゲン複合体スーパーファミリーのメンバーです。[1]COL1A1は、フォン・ヴィレブランド因子C型（vWFC）ドメイン、コラーゲン三重らせんの反復、および線維状コラーゲンC末端ドメイン（COLF）を有します。[2]
Application
Biochem/physiol Actions
Physical form
0.02N酢酸、pH 3.67
Preparation Note
4°Cで1年間
Analysis Note
均一なゲル化の試験と、PC-12ラット細胞の接着および拡散の試験で常に評価されています。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
メルクのカタログまたは製品に添付されたメルクのその他の文書に記載されていない場合、メルクの製品は研究用途のみを目的としているため、他のいかなる目的にも使用することはできません。このような目的としては、未承認の商業用途、in vitroの診断用途、ex vivoあるいはin vivoの治療用途、またはヒトあるいは動物へのあらゆる種類の消費あるいは適用などがありますが、これらに限定されません。
1 of 1
当該品目 | |||
|---|---|---|---|
| biological source rat | biological source rat tail | biological source human skin | biological source rabbit |
| technique(s) cell culture | mammalian: suitable (and for 3D proliferation assays.) | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) ELISA: suitable, immunohistochemistry: suitable, radioimmunoassay: suitable, western blot: suitable |
| concentration 100 mg | concentration - | concentration - | concentration - |
| Quality Level 100 | Quality Level - | Quality Level 100 | Quality Level 100 |
| form liquid, solution | form lyophilized (clear, colorless solution after reconstitution) | form lyophilized | form - |
| Gene Information rat ... COL1A1(29393) | Gene Information rat ... Col1a1(29393) | Gene Information - | Gene Information human ... COL1A1(1277) |
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保管分類
12 - Non Combustible Liquids
wgk
WGK 1
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
プロトコル
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
資料
ラミニン、コラーゲン、フィブロネクチンなどの細胞外基質タンパク質は細胞培養の際の細胞接着基質として使用されます。
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
M Yebra et al.
The Journal of biological chemistry, 271(46), 29393-29399 (1996-11-15)
The urokinase plasminogen activator (uPA) interacts with its cell surface receptor (uPAR), providing an inducible, localized cell surface proteolytic activity, thereby promoting cellular invasion. Evidence is provided for a novel function of cell surface-associated uPA.uPAR. Specifically, induction of cell surface
Kerim B Kaylan et al.
Scientific reports, 6, 23490-23490 (2016-03-31)
The bipotential differentiation of liver progenitor cells underlies liver development and bile duct formation as well as liver regeneration and disease. TGFβ and Notch signaling are known to play important roles in the liver progenitor specification process and tissue morphogenesis.
C Fink et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 14(5), 669-679 (2000-04-01)
To examine the influence of chronic mechanical stretch on functional behavior of cardiac myocytes, we reconstituted embryonic chick or neonatal rat cardiac myocytes to a 3-dimensional engineered heart tissue (EHT) by mixing freshly isolated cells with neutralized collagen I and
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 08-115 | 08436037123627 |
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Does this product require pH neutralization by addition of acid or base for gelation?
1 回答-
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Hi team: I hope this finds you well. May I know the concentration of collagen type I , rat tail 08-115? Much appreciated. Kind regards, Ovi
1 回答-
The concentration is provided in lot specific Certificates of Analysis, typically within the range of 3 to 5 mg/ml.
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Accidentally, I opened the collagen out of the flow chamber. Should I filter it or autoclave it to ensure the sterile conditions?
1 回答-
Collagen solutions may be sterilized by dialysis in a 0.25% acetic acid and 0.5% chloroform solution.
Avoid sterilizing the collagen solution by membrane filtration, as substantial protein loss may occur by this method. The coating of tissue culture plastic dishes may be performed by air-drying. Dried coated dishes may then be sterilized by exposure to UV light in a sterile culture hood or by rinsing with 70% ethanol.役に立ちましたか？
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Hi, what is the concentration of the collagen?
1 回答-
The concentration of this product will be listed on the lot-specific Certificate of Analysis. Please see the link below to access a Certificate:
https://www.emdmillipore.com/US/en/product/Collagen-Type-I-rat-tail,MM_NF-08-115#anchor_COA役に立ちましたか？
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Once diluted in 0.02N acetic acid, does the collagen solution require sterilisation for use in cell culture? If so, which method is the most effective: UV, autoclave or 0.22uM sterile filter (should this be PES or PVDF)?
1 回答-
This product is supplied as a sterile solution in 0.02 N Acetic acid. The package size is 100 mg, however, the concentration is variable and reported on the lot-specific Certificate of Analysis. The typical range is 4 - 5 mg/mL. Dilutions can be made with sterile PBS. Further sterilization is not required. If necessary, coated slides and coverslips can be sterilized by UV.
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