E4013

モノクロナール抗エラスチン マウス宿主抗体

clone BA-4, ascites fluid

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00164587
• Conjugate: unconjugated
• Clone: BA-4, monoclonal
• Application: ARR, IHC (p)
• Citations: 29

特性

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: BA-4, monoclonal
• mol wt: antigen 67.5 kDa
• contains: 15 mM sodium azide
• species reactivity: human, goat, sheep, guinea pig, pig, canine, feline, bovine
• packaging: antibody small pack of 25 μL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:5,000 using sheep arterial tissue, microarray: suitable
• isotype: IgG1
• UniProt accession no.: P15502
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ELN(2006)

詳細

General description

Monoclonal Anti-Elastin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Elastin is an important polymeric protein of connective tissue. It is synthesized and secreted as a soluble single-chain protein (tropoelastin) that undergoes numerous post-ribosomal modifications prior to the organization of the elastic fiber in the extracellular space.

Immunogen

ウシα-エラスチン

Application

Monoclonal Anti-Elastin antibody produced in mouse has been used in:

• immunohistochemistry
• immunostaining
• immunoblotting

Biochem/physiol Actions

本抗体は不溶性エラスチン、α-エラスチン、トロポエラスチン (エラスチンの非架橋可溶性前駆体) 、不溶性エラスチンのタンパク質分解で生じるペプチドフラグメントに結合します。トロポエラスチ調製物の場合、エラスチンの3つのアイソフォ-ムに対応する3本のバンド (67.5kDa, 65kDa, 62kDa) を検出します。この抗体はエラスチンの繰返し配列から構成されるエピト-プ (Val-Gly-Val-Ala-Pro-Gly) に特異的です。このエピト-プは線維芽細胞および単球に対する化学誘引物質であることが示されています。

Elastin is encoded by the ELN gene in humans. It is a polymeric protein in connective tissue that has exceptional properties of extension, elastic recoil to the extracellular matrix and allows many tissues in the body to resume their shape after stretching or contracting. Elastin is a self-assembling, extracellular-matrix protein providing tissue elasticity and is predominantly comprised of cross-linked tropoelastin. It plays an important role in the atherosclerotic process. The conformational disorder is caused due to a constitutive feature of elastin structure and function.

Elastin undergoes changes of morphology with aging and in many disease states. For example, abnormal accumulation of connective tissue in blood vessels contributes to alterations in vascular physiology associated with diseases such as hypertension and atherosclerosis. Several heritable diseases such as Marfan syndrome, pseudoxanthoma elasticum and the Buschke-Ollendorf syndrome may result from molecular defects of elastin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable