D3071

ジペプチジルペプチダーゼIX ヒト

recombinant, expressed in Sf9 cells

• NACRES: NA.54
• UNSPSC Code: 12352204
• Specific activity: ≥250 units/μg protein
• Recombinant: expressed in Sf9 cells

特性

• recombinant: expressed in Sf9 cells
• Quality Segment: 200
• form: solution
• specific activity: ≥250 units/μg protein
• shipped in: dry ice
• storage temp.: −70°C

詳細

General description

N末端GST融合完全長塩基配列

Application

DPP9 is a protease of the DPP4 gene family that is used in cancer research to study the regulation of survival and proliferation pathways[1]. DPP9 is used to study cell-extracellular matrix interactions and tissue remodeling[2].

Biochem/physiol Actions

DPP IX is a member of the S9b family of dipeptidyl peptidases and cleaves the peptide hormones glucagon-like peptide-1, glucagon-like peptide-2, neuropeptide Y and peptide YY with marked kinetic differences compared with dipeptidyl peptidase IV. It has a role in regulating cell survival and proliferation through modulating molecular signaling cascades. Protein kinase B activation is significantly inhibited by human DPP9 overexpression in human hepatoma cells (HepG2 and Huh7) and human embryonic kidney cells (HEK293T)[1]. DPP9 is thought to influence cell-extracellular matrix interactions and may be involved in the regulation of tissue remodeling [2].

Physical form

25 mM Tris-HCl(pH 8.0)、100 mM NaCl、0.05% Tween-20、50% グリセロール、3 mM DTTで調製した溶液。

Other Notes

1 unitは、pH 7.5、25°C、1分間に、1.0 pmolのH-Gly-Pro-AMCを加水分解する酵素量です。

DPP IXは、S9bジペプチジルペプチダーゼファミリーのメンバーです。ペプチドホルモンであるグルカゴン様ペプチド-1、グルカゴン様ペプチド-2、神経ペプチドY、ペプチドYYを切断します。ジペプチジルペプチダーゼIVと比べて、動態が大きく異なっています。

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 1