D0571

モノクローナル抗DICER1抗体 マウス宿主抗体

~1.0 mg/mL, clone DCR1, purified immunoglobulin, buffered aqueous solution

• 別名: 抗DCR1抗体, 抗HERNA抗体, 抗K12H4.8-LIKE抗体, 抗ヘリカーゼ-MOI抗体, 抗ヘリカーゼ/RNアーゼモチーフ抗体
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: DCR1, monoclonal
• Application: WB
• Citations: 9

特性

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: DCR1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~218 kDa
• species reactivity: human, mouse, rat
• concentration: ~1.0 mg/mL
• technique(s): western blot: 2.5-5.0 μg/mL using whole extract of human HEK-293T cells
• isotype: IgG1
• UniProt accession no.: Q9UPY3
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DICER1(23405) ; mouse ... Dicer1(192119) ; rat ... Dicer1(299284)

詳細

General description

Dicer is a member of the RNase III family containing an N-terminal DEXH-box RNA helicase/ATPase domain, followed by a domain of unknown function (DUF283), a PAZ domain, which anchors the 3′-end of the guided siRNA, two RNase III domains, and a dsRBD. The current model for dsRNA cleavage by Dicer predicts that the two ribonuclease III domains of Dicer dimerize to form the catalytic center that is responsible for cleaving long dsRNA in co-operation with two RNA binding domains, PAZ and dsRNA-binding domain.

Monoclonal Anti-DICER1 (mouse IgG1 isotype) is derived from the hybridoma DCR1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein corresponding to a fragment of human DICER1.

Application

The antibody may be used in several immunological techniques including immunoblotting and real‐time PCR.

Biochem/physiol Actions

Dicer catalyzes the first step in the RNA interference (RNAi) pathway and initiates formation of the RNA-induced silencing complex (RISC). The dsRNA is processed into small fragments called short interfering RNA (siRNA) or microRNA (miRNA) of typically 21-25 nucleotides long with a two-base overhang on the 3′ end. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. DICER1-deficient colorectal cancer cells have an enhanced ability to initiate tumors and metastasis. Dicer functions as an indirect tumor suppressor, as silencing Dicer expression makes cancer cells more malignant.

Physical form

0.01 M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable