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由来生物
mouse
結合体
unconjugated
抗体製品の状態
purified from hybridoma cell culture
抗体製品タイプ
primary antibodies
クローン
CY-96, monoclonal
形状
buffered aqueous solution
濃度
~1.5 mg/mL
テクニック
direct ELISA: suitable
dot blot: 1-2 μg/mL using cell protein extracts labeded with Cy3 or Cy5
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
アイソタイプ
IgG2a
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
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詳細
Anti-Cy3/Cy5 antibody, Mouse monoclonal, (mouse IgG2a isotype) is derived from the hybridoma CY-96 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from Balb/c mice immunized with a mixture of proteins labeled with Cy3 or Cy5.
特異性
抗体は、Cy3, Cy5, AlexaFluro 647, AlexaFluor 547標識されたタンパク質を認識します。
免疫原
Cy3またはCy5標識タンパク質の混合物。
アプリケーション
Anti-Cy3/Cy5 antibody, Mouse monoclonal has been used in:
- immunofluorescence
- western blot
- dot blot
- enzyme linked immunosorbent assay (ELISA)
- immunoprecipitation
- immunocytochemistry
- protein microarrays
- In in situ hybridization
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
生物化学的／生理学的作用
Cy 3 and Cy5 are the most popular cyanine dyes, used combined for two color detection. Cy3 dyes are fluorescent orange while Cy5 is fluorescent in the red region. Cyanine belonging to polymethine group. CyDyes are a family of fluorophores that can be used for labeling proteins, peptides, DNA, RNA, and other biomolecules. These dyes are small, pH insensitive, soluble in aqueous solution and are tolerant to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. Mainly Cy3 and Cy5 are used in many different biological assays such as DNA microarrays, protein microarrays, two-dimensional protein analysis (2D gels), fluorescence resonance energy transfer (FRET), and immunocytochemistry.
物理的形状
0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
C0992-VAR:
IXO97101:
C0992-BULK:
C0992-200UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection
Nagai-Okatani C, et al.
International Journal of Molecular Sciences, 20(3), 700-700 (2019)
Transplantation of ovarian granulosa-like cells derived from human induced pluripotent stem cells for the treatment of murine premature ovarian failure
Liu T. et al.
Molecular Medicine Reports, 13(6), 5053-5058 (2016)
G Enders
Acta neurochirurgica. Supplement, 89, 9-13 (2004-09-01)
Microarray analysis has been emerged as a tool to characterize the overall reaction of cells in culture or tissue to different stimuli e.g. stressful events by analysing bulk RNA present at a particular time point. It has supplemented or even
Chiaki Nagai-Okatani et al.
International journal of molecular sciences, 20(3) (2019-02-10)
Lectin microarray (LMA) is a highly sensitive technology used to obtain the global glycomic profiles of endogenous glycoproteins in biological samples including formalin-fixed paraffin-embedded tissue sections. Here, we describe an effective method for cell type-selective glycomic profiling of tissue fragments
A K Kenworthy
Methods (San Diego, Calif.), 24(3), 289-296 (2001-06-14)
Fluorescence resonance energy transfer (FRET) detects the proximity of fluorescently labeled molecules over distances >100 A. When performed in a fluorescence microscope, FRET can be used to map protein-protein interactions in vivo. We here describe a FRET microscopy method that
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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