B0271

モノクローナル抗β-ガラクトシダーゼ抗体－ビオチン標識 マウス宿主抗体

clone GAL-13, purified immunoglobulin, buffered aqueous solution

• 別名: モノクローナル抗β-ガラクトシダーゼ抗体
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: biotin conjugate
• Clone: GAL-13, monoclonal
• Application: DB
• Citations: 7

特性

• biological source: mouse
• Quality Level: 200
• conjugate: biotin conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: GAL-13, monoclonal
• form: buffered aqueous solution
• species reactivity: bacteria
• technique(s): dot blot: 1:2,000
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

詳細

General description

Monoclonal Anti-b-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. β-Galactosidase enzyme is encoded by lacZ gene of E. coli.

Immunogen

βE. coli由来の-ガラクトシダーゼ

Application

Monoclonal Anti-β-Galactosidase-Biotin Conjugate antibody produced in mouse may be used with ExtrAvidin-Peroxidase in the dot blot technique on native, purified, or crude β-galactosidase. The antibody was used to detect β-galactosidase by immunofluorescence in paraffin-embedded adult mouse kidney sections. It was used in ELISPOT assays at a dilution of 1:1000.

Biochem/physiol Actions

本抗体は、β-ガラクトシダーゼ抗-β-ガラクトシダーゼ（BGABG）可溶性複合体の調製による免疫酵素染色における増殖に使用できます。本抗体は、免疫ブロッティングにはお勧めしません。本抗体はβ-ガラクトシダーゼの変性または減少を認識しません。

β-galactosidase enzyme is encoded by lacZ gene of E. coli; it acts on lactose and cleaves it to glucose and galactose. Monoclonal Anti-β-Galactosidase-Biotin Conjugate antibody binds with soluble enzyme even when bound to a surface without causing any loss of enzymatic activity. It is used as readout for promoter activity in lacZ transfected cells.

Physical form

0.01 M PBS溶液(pH 7.4, 1% BSA, 15 mMアジ化ナトリウム含有)。

Preparation Note

ε-アミノカプロイルビオチンとのコンジュゲ-ションにより作製しています。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable