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TMKIT

mPAGE®TurboMix Bis-Tris Gel Casting Kit

Premixed solutions for casting bis-tris polyacrylamide gels; TMKIT-10 includes 36 mL resolving solution and 20 mL stacking solution; TMKIT-60 includes 216 mL resolving solution and 120 mL stacking solution

別名:

Bis-Tris gel casting kit, SDS-PAGE gel casting kit, gel casting kit, protein gel casting kit

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この商品について

NACRES:
NA.25
UNSPSC Code:
41105319
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Quality Segment

sterility

0.2 μm filtered

form

liquid

feed ratio

29:1

storage condition

protect from light

storage temp.

2-8°C

General description

mPAGE® TurboMix Bis-Tris Gel Casting Kits deliver all the benefits of bis-tris gel chemistry. The gels operate at neutral pH which minimizes protein modification and promotes protein stability during the run. This leads to sharper bands and improved resolution. Run times are about 40% faster than standard tris-glycine gels. Cast gels can be stored at 4 °C for up to 4 weeks and are compatible with standard staining and transfer methods.
The mPAGE® TurboMix casting workflow offer many improvements over standard processes.

  • Pre-mixed acrylamide buffer solutions save time and improve reproducibility
  • Flexibility: dilute resolving solution with DI water to any gel percentage between 8% and 15%
  • Quick Cast protocol allows simultaneous polymerization of stacking and resolving gels, saving even more time

Application

Premixed solutions for casting bis-tris polyacrylamide gels.

Legal Information

MPAGE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.




試験成績書(COA)

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プロトコル

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

メルクのプレミックス溶液とプロトコルでSDS-PAGEゲル調製を簡素化し、正確なタンパク質分離を達成しましょう。

資料

迅速かつ確実なゲルキャスティングとタンパク質分離のためにmPAGE® TurboMixテクノロジーを用いるBis-Tris SDS-PAGEゲルキャスティングキット。

Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.

関連コンテンツ

We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.

我々はそれを理解している。ウエスタンはしみ抜きで苦労することがある。そこで、あなたの醜いブロットを私たちと共有し、サプライズを受け取ってください!それが何であるかは言えませんが-それは驚きを台無しにしてしまうからです-ラボの創造性を掻き立てるということを除いては。






質問

1–3/3 質問  
  1. In TMKIT mPAGE kit details 1. Flexibility: dilute resolving solution with DI water to any gel percentage between 8% and 15% Could you please confirm the stock resolving solution percentage? 2. Feed ratio 29:1 If the feed ratio indicates the ratio

    1 回答
    1. This kit may be used to prepare gels at a range of 8 - 15%. An 8% gel requires 2.4 mL Resolving Solution, a 10% requires 3 mL, a 12% requires 3.6 mL, and a 15% requires 4.5 mL. See the table below for additional details. The feed ratio refers to the amount of acrylamide monomer to crosslinker.

      Additional information may be accessed in the Product User Guide at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/132/613/20508849w-ms.pdf

      役に立ちましたか?

  2. What is the composition of the resolving (TMXR-36ML) and stacking (TMXS-25ML) solutions that are apart of the kit TMKIT?

    1 回答
    1. The composition is considered propreietary.

      役に立ちましたか?

  3. Is SDS present in the resolving (TMXR-36ML) and stacking (TMXS-25ML) solutions that are apart of the kit?

    1 回答
    1. SDS is not present in the formulation.

      役に立ちましたか?

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