MABE251

Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31

clone HMC 31, from mouse

• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: HMC 31, monoclonal
• 交差性（ホモロジーによる予測）: all
• テクニック: ELISA: suitable; dot blot: suitable; methylated DNA immunoprecipitation (MeDIP): suitable
• アイソタイプ: IgG1κ
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammilian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.

特異性

This antibody recognizes the 5-hydroxymethylcytosine of 5-Hydroxymethylcytosine DNA.

免疫原

BSA-conjugated 5-hydroxymethylcytosine.

Epitope: 5-hydroxymethylcytosine

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
エピジェネティクス

Detect 5-hydroxymethylcytosine (5hmC) with Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31 (Mouse Monoclonal Antibody), that has been shown to work in DB, MeDIP, ELISA.

ELISA Analysis: A representative lot detected 5-hydroxymethylcytosine in ELISA.

Methylated DNA Immunoprecipitation (MeDIP) Analysis: A representative lot immunoprecipitated 5-hydroxymethylcytosine in ChIP.

品質

Evaluated by Dot Blot in unmodified and modified cytosine DNA.

Dot Blot Analysis: 0.1 µg/mL of this antibody detected 5-hydroxymethylcytosine in 10 ng and 50 ng of unmodified and modified cytosine DNA.

ターゲットの説明

This antibody detects 5-hydroxymethylcytosine.

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Unmodified and modified cytosine DNA.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable