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Instructions For Use
Coating Procedure
Note:Use these recommendations as guidelines to determine the optimal coating conditions for your culture system. The bottom of the plate can be detached by excessive mechanical force such as centrifugation or direct contact with liquid handling tools (tips, pipettes, etc.).
1. Remove the CytoSoft® product from the protective sleeve in a sterile hood.
2. Prepare extracellular matrix material by neutralizing in amine-free buffer pH 7.4 to 7.9 (such as 1X DPBS). We do not recommend using gelatin as your ECM protein. Note: Pre-warm the coating solution to approximately room temperature before use.
3. Dilute as needed, and dispense 1 mL of solution into each well to coat the surface. Note: Recommended dilution for PureCol™ Type I collagen is 1:30 (~100 µg/ml). Note: The hydrophobic surface requires larger volumes to cover the surface than do conventional plastic dishes
4. Incubate ECM coated CytoSoft® at room temperature, covered for 1 hour.
5. After incubation, aspirate any remaining material and rinse coated surfaces immediately two times with culture medium or PBS. Leave about 2.5 mL of medium per well to keep surface covered. Note: Do not allow the CytoSoft® surface to become dry once the surface has been wetted.
6. Coated surfaces are ready for use. Standard harvesting procedures used for removing cells from cultureware can be employed for harvesting cells from the CytoSoft® product including use of trypsin, Accutase® and non-enzymatic cell detachment solutions.
Coating Procedure
Note:Use these recommendations as guidelines to determine the optimal coating conditions for your culture system. The bottom of the plate can be detached by excessive mechanical force such as centrifugation or direct contact with liquid handling tools (tips, pipettes, etc.).
1. Remove the CytoSoft® product from the protective sleeve in a sterile hood.
2. Prepare extracellular matrix material by neutralizing in amine-free buffer pH 7.4 to 7.9 (such as 1X DPBS). We do not recommend using gelatin as your ECM protein. Note: Pre-warm the coating solution to approximately room temperature before use.
3. Dilute as needed, and dispense 1 mL of solution into each well to coat the surface. Note: Recommended dilution for PureCol™ Type I collagen is 1:30 (~100 µg/ml). Note: The hydrophobic surface requires larger volumes to cover the surface than do conventional plastic dishes
4. Incubate ECM coated CytoSoft® at room temperature, covered for 1 hour.
5. After incubation, aspirate any remaining material and rinse coated surfaces immediately two times with culture medium or PBS. Leave about 2.5 mL of medium per well to keep surface covered. Note: Do not allow the CytoSoft® surface to become dry once the surface has been wetted.
6. Coated surfaces are ready for use. Standard harvesting procedures used for removing cells from cultureware can be employed for harvesting cells from the CytoSoft® product including use of trypsin, Accutase® and non-enzymatic cell detachment solutions.
CytoSoft® T-25 flasks have a defined elastic modulus in the bottom of a standard T-25 flask for cell expansion applications. The thickness of the silicone gel is uniform with a ~0.5 mm thick layer of silicone in each flask that is fully compatible with mammalian cell cultures. The silicone gels are activated and ready to bind to a purified ECM, such as PureCol™ type I collagen (#5005) prior to cell addition. The plates are packaged sterilized, and provided with 10 flasks per package.
The rigidity of the substrate to which cells adhere can have a profound effect on cell morphology and gene expression. CytoSoft® products provide a tool to culture cells on substrates with various rigidities covering a broad physiological range. On the bottom of each flask, there is a thin layer of specially formulated biocompatible silicone, whose elastic modulus (rigidity) is carefully measured and certified. The surfaces of the gels in CytoSoft® products are functionalized to form covalent bonds with amines on proteins. This chemical functionalization is stable and the reaction does not require a catalyst, facilitating the coating of the gel surfaces with matrix proteins and plating cells.
The silicone substrates of CytoSoft® products are optically clear and have a low auto-florescence. The layer of silicone in each flask is firmly bonded to the bottom of the flask. Unlike hydrogels (such as polyacrylamide gels), silicone gels are not susceptible to hydrolysis, do not dry nor swell, are resilient and resistant to tearing or cracking, and their elastic moduli (rigidities) remain nearly unchanged during extended storage periods.
CytoSoft® products accommodate the harvesting of cells using enzymes such as trypsin and collagenase. There is no biochemical breakdown of the substrate during or after enzyme treatment, and there are no residuals of the substrate in the sample retrieved from a CytoSoft® plate.
The rigidity of the substrate to which cells adhere can have a profound effect on cell morphology and gene expression. CytoSoft® products provide a tool to culture cells on substrates with various rigidities covering a broad physiological range. On the bottom of each flask, there is a thin layer of specially formulated biocompatible silicone, whose elastic modulus (rigidity) is carefully measured and certified. The surfaces of the gels in CytoSoft® products are functionalized to form covalent bonds with amines on proteins. This chemical functionalization is stable and the reaction does not require a catalyst, facilitating the coating of the gel surfaces with matrix proteins and plating cells.
The silicone substrates of CytoSoft® products are optically clear and have a low auto-florescence. The layer of silicone in each flask is firmly bonded to the bottom of the flask. Unlike hydrogels (such as polyacrylamide gels), silicone gels are not susceptible to hydrolysis, do not dry nor swell, are resilient and resistant to tearing or cracking, and their elastic moduli (rigidities) remain nearly unchanged during extended storage periods.
CytoSoft® products accommodate the harvesting of cells using enzymes such as trypsin and collagenase. There is no biochemical breakdown of the substrate during or after enzyme treatment, and there are no residuals of the substrate in the sample retrieved from a CytoSoft® plate.
アプリケーション
Young′s elastic modulus: 26.0-38.0 kPa
Sterility: Ozone
Cell attachment: Tested
Cell growth: Tested
Sterility: Ozone
Cell attachment: Tested
Cell growth: Tested
保管および安定性
Store CytoSoft® Imaging plates at room temperature.
その他情報
Elastic Moduli 32 kPa
法的情報
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CytoSoft is a registered trademark of Advanced BioMatrix, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
13 - Non Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
労働安全衛生法名称等を表示すべき危険物及び有害物
名称等を表示すべき危険物及び有害物
労働安全衛生法名称等を通知すべき危険物及び有害物
名称等を通知すべき危険物及び有害物
Jan Code
CC335:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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